PMID- 12723927
OWN - NLM
STAT- MEDLINE
DCOM- 20030714
LR  - 20191210
IS  - 1060-3271 (Print)
IS  - 1060-3271 (Linking)
VI  - 86
IP  - 2
DP  - 2003 Mar-Apr
TI  - Comparative tests to improve the gas chromatographic analysis of chlorobornanes 
      in fish samples.
PG  - 432-8
AB  - A comparison was made between electron capture negative ionization quadrupole 
      mass spectrometry (ECNI-MS) and electron capture detection (ECD) with regard to 
      repeatability and reproducibility for the gas chromatographic (GC) analysis of 
      toxaphene congeners [chlorobornanes (CHBs)]. The tests, including standard 
      solutions and several cleaned fish extracts, showed larger relative standard 
      deviations (RSDs) for the repeatability of ECNI-MS but no differences in the 
      reproducibility of the 2 techniques. The sensitivity of the GC-ECNI-MS was 
      considerably better than that of GC/ECD. Four stepwise-designed comparative tests 
      were also conducted on GC analysis, cleanup, and the complete method. The results 
      showed that, according to the current state-of-the-art, coefficients of variation 
      for the between-laboratory performance were not < 20% and were usually between 20 
      and 30%. In spite of separation problems, e.g., for CHB 26, which cannot be 
      separated into a single-component peak, a 95% methyl 5% phenyl polysiloxane (CP 
      Sil 8) column was preferred to more polar columns for the analysis of CHBs 26, 
      40, 41, 44, 50, and 62. CHB 62 was more difficult to determine than CHB 26 and 
      50. Addition of the CHBs 40, 41, and 44 to the standard set of 3 chlorobornanes 
      (26, 50, and 62) resulted in more separation problems. A 3-step cleanup method 
      was recommended.
FAU - de Boer, Jacob
AU  - de Boer J
AD  - Netherlands Institute for Fisheries Research, PO Box 68, 1970 AB IJmuiden, The 
      Netherlands. j.deboer@rivo.wag-ur.nl
FAU - Leonards, Pim E G
AU  - Leonards PE
FAU - Klungsoyr, Jarle
AU  - Klungsoyr J
FAU - McHugh, Brendan
AU  - McHugh B
FAU - Nixon, Eugene
AU  - Nixon E
FAU - McGovern, Evin
AU  - McGovern E
FAU - Rimkus, Gerhard G
AU  - Rimkus GG
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - J AOAC Int
JT  - Journal of AOAC International
JID - 9215446
RN  - 0 (Camphanes)
RN  - 0 (Fish Oils)
RN  - 0 (Insecticides)
RN  - 0 (Pesticide Residues)
RN  - 8001-35-2 (Toxaphene)
SB  - IM
MH  - Animals
MH  - Camphanes/*analysis
MH  - Chromatography, Gas
MH  - Electrochemistry
MH  - Fish Oils/analysis
MH  - Fishes/*metabolism
MH  - Insecticides/*analysis
MH  - Mass Spectrometry
MH  - Pesticide Residues/*analysis
MH  - Reference Standards
MH  - Reproducibility of Results
MH  - Toxaphene/*analysis
EDAT- 2003/05/02 05:00
MHDA- 2003/07/15 05:00
CRDT- 2003/05/02 05:00
PHST- 2003/05/02 05:00 [pubmed]
PHST- 2003/07/15 05:00 [medline]
PHST- 2003/05/02 05:00 [entrez]
PST - ppublish
SO  - J AOAC Int. 2003 Mar-Apr;86(2):432-8.