PMID- 12724286
OWN - NLM
STAT- MEDLINE
DCOM- 20040406
LR  - 20171116
IS  - 0006-3363 (Print)
IS  - 0006-3363 (Linking)
VI  - 69
IP  - 2
DP  - 2003 Aug
TI  - Human choriocarcinoma cell resistance to natural killer lysis due to defective 
      triggering of natural killer cells.
PG  - 627-33
AB  - The trophoblast, the outermost layer of the human placenta, lacks expression of 
      the classical human leukocyte antigen (HLA) class I molecules. This prevents 
      allorecognition by T cells but raises the question of what protects the 
      trophoblast from natural killer (NK) cells. In a previous study, we have shown 
      that choriocarcinoma cell (CC) resistance to NK lysis was mainly independent of 
      HLA class I molecules. In the present study, we postulated that CC may prevent 
      activation of NK cells by failing to stimulate their triggering receptors (TR). 
      To test this hypothesis, we evaluated the lysis of JAR and JEG-3 CC after 
      effective cross-linking and activation of NK cells by means of lectins or 
      antibodies. Our results show that NK-resistant CC were sensitive to lysis by 
      unstimulated peripheral blood lymphocytes in the presence of phytohemagglutin 
      (PHA), to antibody-dependent cell cytotoxicity in presence of anti-Tja 
      antibodies, and to monoclonal antibody redirected killing using anti-TR 
      antibodies anti-CD16 and anti-CD244/2B4. Finally, CC fail to express CD48, the 
      ligand for CD244/2B4. These results indicate that the resistance of CC to lysis 
      results primarily from defective NK cell activation, at least partially due to 
      the lack of expression of ligands, such as CD48, involved in the triggering of NK 
      cells.
FAU - Avril, Tony
AU  - Avril T
AD  - EA 3249 Cellules hematopoietiques, hemostase et greffe, Faculte de Medecine, 
      Tours, France.
FAU - Iochmann, Sophie
AU  - Iochmann S
FAU - Brand, Denys
AU  - Brand D
FAU - Bardos, Pierre
AU  - Bardos P
FAU - Watier, Herve
AU  - Watier H
FAU - Thibault, Gilles
AU  - Thibault G
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20030430
PL  - United States
TA  - Biol Reprod
JT  - Biology of reproduction
JID - 0207224
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (Antigens, CD)
RN  - 0 (CD2 Antigens)
RN  - 0 (CD244 protein, human)
RN  - 0 (CD48 Antigen)
RN  - 0 (CD48 protein, human)
RN  - 0 (DNA, Complementary)
RN  - 0 (Fc gamma receptor IIA)
RN  - 0 (Membrane Glycoproteins)
RN  - 0 (Phytohemagglutinins)
RN  - 0 (Receptors, IgG)
RN  - 0 (Receptors, Immunologic)
RN  - 0 (Signaling Lymphocytic Activation Molecule Family)
SB  - IM
MH  - Antibodies, Monoclonal/pharmacology
MH  - Antibody-Dependent Cell Cytotoxicity/physiology
MH  - Antigens, CD/biosynthesis/genetics
MH  - CD2 Antigens/genetics
MH  - CD48 Antigen
MH  - Cell Line, Tumor
MH  - Choriocarcinoma/*pathology
MH  - Cytotoxicity, Immunologic
MH  - DNA, Complementary/genetics
MH  - Fluorescent Antibody Technique
MH  - Humans
MH  - Killer Cells, Natural/*physiology
MH  - Membrane Glycoproteins/genetics
MH  - Phytohemagglutinins/pharmacology
MH  - Receptors, IgG/genetics
MH  - Receptors, Immunologic/genetics
MH  - Signaling Lymphocytic Activation Molecule Family
MH  - Transfection
EDAT- 2003/05/02 05:00
MHDA- 2004/04/07 05:00
CRDT- 2003/05/02 05:00
PHST- 2003/05/02 05:00 [pubmed]
PHST- 2004/04/07 05:00 [medline]
PHST- 2003/05/02 05:00 [entrez]
AID - biolreprod.102.009290 [pii]
AID - 10.1095/biolreprod.102.009290 [doi]
PST - ppublish
SO  - Biol Reprod. 2003 Aug;69(2):627-33. doi: 10.1095/biolreprod.102.009290. Epub 2003 
      Apr 30.