PMID- 12726713
OWN - NLM
STAT- MEDLINE
DCOM- 20040408
LR  - 20220321
IS  - 0142-9612 (Print)
IS  - 0142-9612 (Linking)
VI  - 24
IP  - 15
DP  - 2003 Jul
TI  - Comparison of human bone marrow stromal cells seeded on calcium-deficient 
      hydroxyapatite, beta-tricalcium phosphate and demineralized bone matrix.
PG  - 2593-603
AB  - The aim of this study was to compare three resorbable biomaterials regarding 
      seeding efficacy with human bone marrow stromal cells (BMSCs), cell penetration 
      into the matrix, cell proliferation and osteogenic differentiation. 
      Calcium-deficient hydroxyapatite (CDHA), beta-tricalcium phosphate (beta-TCP), 
      and demineralized bone matrix (DBM) were seeded with human BMSCs and kept in 
      human serum and osteogenic supplements for 3 weeks. Morphologic and biochemical 
      evaluations were performed on day 1, 7, 14 and 21. The allograft DBM and CDHA 
      exhibited both an excellent seeding efficacy while the performance of beta-TCP 
      was lower when compared. The total protein content and the values for specific 
      alkaline phosphatase (ALP) increased on all matrices and no significant 
      difference was found for these two markers. BMSCs in monolayer had a significant 
      increase of protein, but not of ALP. Osteocalcin (OC) values increased 
      significantly higher for BMSC in cultures on DBM when compared to CDHA and 
      beta-TCP. The OC levels decreased significantly in the BMSC monolayer culture. 
      BMSCs were found inconsistently within the synthetic materials, whereas in DBM 
      they were found more homogeneously distributed throughout the matrix. All three 
      matrices promoted BMSC proliferation and differentiation to osteogenic cells. DBM 
      allografts seem to be more favorable with respect to cell ingrowth tested by 
      histology, and osteogenic differentiation ascertained by an increase of OC. CDHA 
      with its high specific surface area showed more favorable properties than 
      beta-TCP regarding reproducibility of the seeding efficacy.
FAU - Kasten, P
AU  - Kasten P
AD  - Orthopedic Surgery, University of Heidelberg, Schlierbacher Landstr. 200a, 69118, 
      Heidelberg, Germany. philip.kasten@gmx.de
FAU - Luginbuhl, R
AU  - Luginbuhl R
FAU - van Griensven, M
AU  - van Griensven M
FAU - Barkhausen, T
AU  - Barkhausen T
FAU - Krettek, C
AU  - Krettek C
FAU - Bohner, M
AU  - Bohner M
FAU - Bosch, U
AU  - Bosch U
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PL  - Netherlands
TA  - Biomaterials
JT  - Biomaterials
JID - 8100316
RN  - 0 (Biocompatible Materials)
RN  - 0 (Calcium Phosphates)
RN  - 0 (Hydroxyapatites)
RN  - 0 (beta-tricalcium phosphate)
RN  - 104982-03-8 (Osteocalcin)
RN  - K4C08XP666 (tricalcium phosphate)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - Biocompatible Materials/metabolism
MH  - Bone Marrow Cells/*metabolism/ultrastructure
MH  - Bone Matrix/*metabolism
MH  - Calcium/*metabolism
MH  - Calcium Phosphates/*metabolism
MH  - Cell Culture Techniques/methods
MH  - Cell Differentiation
MH  - Cell Division
MH  - Cells, Cultured
MH  - Humans
MH  - Hydroxyapatites/*metabolism
MH  - Materials Testing
MH  - Osteocalcin/metabolism
MH  - Stromal Cells/*metabolism/ultrastructure
EDAT- 2003/05/03 05:00
MHDA- 2004/04/09 05:00
CRDT- 2003/05/03 05:00
PHST- 2003/05/03 05:00 [pubmed]
PHST- 2004/04/09 05:00 [medline]
PHST- 2003/05/03 05:00 [entrez]
AID - S0142961203000620 [pii]
AID - 10.1016/s0142-9612(03)00062-0 [doi]
PST - ppublish
SO  - Biomaterials. 2003 Jul;24(15):2593-603. doi: 10.1016/s0142-9612(03)00062-0.