PMID- 12727475
OWN - NLM
STAT- MEDLINE
DCOM- 20040123
LR  - 20061115
IS  - 0966-3274 (Print)
IS  - 0966-3274 (Linking)
VI  - 11
IP  - 1
DP  - 2003 Jan-Mar
TI  - Oncogene expression on the syngeneic beta-cells of long-term surviving pancreatic 
      grafts and better effects of interleukin-1 receptor (IL-1R) and IL-2Rbeta on the 
      grafted beta-cells in LEW/Sea strain rats.
PG  - 49-56
AB  - Thirty-two normal LEW/Sea rats were transplanted a piece of syngeneic pancreas 
      between the peritoneum and abdominal muscle. Among them, 17 (68%) of the 25 rats 
      that received pancreatic transplantation at 41-50 days of age had a surviving 
      beta-cell mass at 5.5-7.1 months after transplantation. Among the 25 rats, 12 
      rats injected with interleukin-1 receptor (IL-1R) and IL-2Rbeta peptides at 
      post-transplantation showed better surviving grafts at 5.5 months' observation. 
      Only 2 (25%) of the other 7 young rats that received a pancreatic graft at 20 
      days of age had a small mass at 21 days post-transplantation. Flow cytometer 
      (FCM) analyses showed that thymus OX40(+) (CD134(+)) T-cells were increased up to 
      37+/-4% at the graft rejection in the 13 old rats without the IL-R peptide 
      injections. The 7 young rats had 99% of thymus OX40(+) T-cells. However, the 12 
      old rats injected with the IL-R peptides showed suppressed numbers of thymus 
      OX40(+) T-cells (8-13+/-3%). The long-term surviving, but apoptotic, grafted 
      beta-cells were stained positively both with anti-insulin monoclonal antibody 
      (mAb) and with anti-c-erbB-2/human epidermal growth factor receptor (HER)-2/neu 
      mAb. Expression of a c-erb family oncogene was shown on the pancreatic graft 
      surviving for 7.1 months. Electron microscopic analysis of the grafted beta-cells 
      showed abnormally large beta granules and loss of functioning mitochondria in the 
      cytoplasm. In 18 (56%) of the 32 rats, the 220-bp and 380-bp specific products of 
      insulin-degrading enzyme (IDE) gene were amplified using the polymerase chain 
      reaction (PCR) of the liver DNA. Among the 18 rats, 6 rats expressed 2 extra 
      hands of 280-bp and 700-bp in a correlation with the high levels of the 
      transforming growth factor-alpha (TGF-alpha) cDNA of 120-bp which was amplified 
      in the quantitative reverse-transcriptase (RT)-PCR of the liver cDNA. Among the 
      selected 11 rats, 5 rats showed large amounts of the 120-bp TGF-alpha cDNA. Host 
      pancreatic RT-PCR showed 235-bp or 250-bp bcl-2 and 181-bp bcl-xS gene products. 
      The bcl-2 cDNA of the host pancreas was amplified actively when the pancreatic 
      graft was being rejected. Exceptionally, the one female injected with the IL-R 
      peptides showed a low level of the liver TGF-alpha cDNA together with the 
      pancreatic expressions of Bax (140-bp), bcl-2 and like interleukin converting 
      enzyme (LICE) (318-bp) cDNA. Insulin secretion from the grafted beta-cells and 
      IL-1beta-induced Fas-mediated apoptosis of the beta-cells were suspected to be 
      present at the same time in the female with the best graft survival.
FAU - Nakatsuji, Tadako
AU  - Nakatsuji T
AD  - Department of Transfusion, Hamamatsu University School of Medicine, 1-20-1 
      Handayama, Hamamatsu 431-3192, Japan. nh80415@hama-med.ac.jp
LA  - eng
PT  - Journal Article
PL  - Netherlands
TA  - Transpl Immunol
JT  - Transplant immunology
JID - 9309923
RN  - 0 (IL2RB protein, human)
RN  - 0 (Il2rb protein, rat)
RN  - 0 (Interleukin-2 Receptor beta Subunit)
RN  - 0 (Receptors, Interleukin)
RN  - 0 (Receptors, Interleukin-1)
RN  - 9007-49-2 (DNA)
SB  - IM
MH  - Animals
MH  - DNA/metabolism
MH  - Female
MH  - Immunohistochemistry
MH  - Interleukin-2 Receptor beta Subunit
MH  - Islets of Langerhans/*immunology/metabolism
MH  - Male
MH  - Microscopy, Electron
MH  - Oncogenes/immunology/*physiology
MH  - Pancreas/pathology/ultrastructure
MH  - Pancreas Transplantation/*immunology
MH  - Rats
MH  - Rats, Inbred Lew
MH  - Receptors, Interleukin/*metabolism
MH  - Receptors, Interleukin-1/*metabolism
MH  - Reverse Transcriptase Polymerase Chain Reaction
EDAT- 2003/05/03 05:00
MHDA- 2004/01/24 05:00
CRDT- 2003/05/03 05:00
PHST- 2003/05/03 05:00 [pubmed]
PHST- 2004/01/24 05:00 [medline]
PHST- 2003/05/03 05:00 [entrez]
AID - S0966-3274(02)00085-0 [pii]
AID - 10.1016/S0966-3274(02)00085-0 [doi]
PST - ppublish
SO  - Transpl Immunol. 2003 Jan-Mar;11(1):49-56. doi: 10.1016/S0966-3274(02)00085-0.