PMID- 12728315
OWN - NLM
STAT- MEDLINE
DCOM- 20030821
LR  - 20181113
IS  - 0945-6317 (Print)
IS  - 0945-6317 (Linking)
VI  - 442
IP  - 6
DP  - 2003 Jun
TI  - Mantle cell lymphoma: improved diagnostics using a combined approach of 
      immunohistochemistry and identification of t(11;14)(q13;q32) by polymerase chain 
      reaction and fluorescence in situ hybridization.
PG  - 538-47
AB  - INTRODUCTION: Mantle cell lymphoma (MCL) is a clinicopathological entity 
      characterized by an aggressive clinical course, morphological features, and 
      overexpression of cyclin D1 due to juxtaposition of the bcl-1 locus (and CCND1 
      gene coding for the cyclin D1) to the IgH gene. This phenomenon is caused by 
      t(11;14)(q13;q32). The morphological diagnosis of MCL may pose difficulties. 
      Ancillary methods are available to support the diagnosis. PATIENTS AND METHODS: 
      We studied a group of 32 patients with MCL; 24 men and 8 women. The median age at 
      the diagnosis was 64 years. We characterized the investigated group by histology, 
      and to analyze the immunohistochemical (IHC) profile we used a panel of 
      antibodies including anti-cyclin D1. Polymerase chain reaction (PCR) was used to 
      detect the rearrangement of bcl-1/IgH in 26 cases (in 11 patients, the DNA was 
      isolated from frozen tissues or from nucleated cells of bone-marrow aspirate or 
      peripheral blood, in 15 patients we utilized paraffin-embedded material). Dual 
      color fluorescence in situ hybridization (FISH) on interphase nuclei detecting 
      the t(11;14)(q13;q32) was applied in all 32 cases. RESULTS: Cyclin D1 IHC was 
      positive in 29 of 30 cases tested (97%). In six, the result was weak and 
      difficult to rely on to support the diagnosis. PCR revealed the fusion gene in 14 
      of the 26 cases (54%). The best yield was obtained from fresh and frozen samples 
      (8 of 11 positive). Using FISH, we identified the translocation in all 32 
      patients, the findings being easily interpretable in 29 patients. In three cases, 
      the intensity of red and green signals was weaker and difficult to read though 
      the co-hybridized signals were identified. The classical pattern of the 
      translocation was observed in 26 patients, while in 3 we found variant patterns 
      suggesting a loss of the V segment of the IgH gene (2x) and a shift in the 
      breakpoint region at chromosome 11 (1x). CONCLUSION: The diagnosis of MCL should 
      be supported by a complex laboratory approach. Interphase FISH seems a useful 
      complementary method to morphology and IHC. It is applicable to various tissues 
      and cells prepared as tissue imprints or histological sections.
FAU - Kodet, Roman
AU  - Kodet R
AD  - Department of Pathology and Molecular Medicine, Charles University, 2nd Medical 
      School and Faculty Hospital in Motol, V Uvalu 84, 150 06, Prague 5-Motol, Czech 
      Republic. Roman.Kodet@lfmotol.cuni.cz
FAU - Mrhalova, Marcela
AU  - Mrhalova M
FAU - Krskova, Lenka
AU  - Krskova L
FAU - Soukup, Jan
AU  - Soukup J
FAU - Campr, Vit
AU  - Campr V
FAU - Neskudla, Tomas
AU  - Neskudla T
FAU - Szepe, Peter
AU  - Szepe P
FAU - Plank, Lukas
AU  - Plank L
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20030501
PL  - Germany
TA  - Virchows Arch
JT  - Virchows Archiv : an international journal of pathology
JID - 9423843
RN  - 0 (Biomarkers, Tumor)
RN  - 0 (DNA, Neoplasm)
RN  - 136601-57-5 (Cyclin D1)
SB  - IM
MH  - Aged
MH  - Aged, 80 and over
MH  - Biomarkers, Tumor/analysis
MH  - *Chromosomes, Human, Pair 11
MH  - *Chromosomes, Human, Pair 14
MH  - Cyclin D1/analysis
MH  - DNA, Neoplasm/analysis
MH  - Female
MH  - Fluorescent Antibody Technique, Indirect
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Lymphoma, Mantle-Cell/chemistry/*diagnosis/genetics
MH  - Male
MH  - Middle Aged
MH  - Polymerase Chain Reaction
MH  - Retrospective Studies
MH  - *Translocation, Genetic
EDAT- 2003/05/03 05:00
MHDA- 2003/08/22 05:00
CRDT- 2003/05/03 05:00
PHST- 2002/11/29 00:00 [received]
PHST- 2003/02/11 00:00 [accepted]
PHST- 2003/05/03 05:00 [pubmed]
PHST- 2003/08/22 05:00 [medline]
PHST- 2003/05/03 05:00 [entrez]
AID - 10.1007/s00428-003-0809-z [doi]
PST - ppublish
SO  - Virchows Arch. 2003 Jun;442(6):538-47. doi: 10.1007/s00428-003-0809-z. Epub 2003 
      May 1.