PMID- 12728455
OWN - NLM
STAT- MEDLINE
DCOM- 20030529
LR  - 20041117
IS  - 0513-5796 (Print)
IS  - 0513-5796 (Linking)
VI  - 44
IP  - 2
DP  - 2003 Apr 30
TI  - Prenatal diagnosis of fetal trisomy 21 from maternal peripheral blood.
PG  - 181-6
AB  - This study was undertaken to establish a noninvasive prenatal genetic diagnostic 
      method for trisomy 21 using the fetal nRBCs that is rarely present in maternal 
      circulation. Peripheral venous blood samples were collected from 30 women with an 
      advanced maternal age, abnormal triple marker test results, or abnormal 
      ultrasound findings such as an increased nuchal translucency. The blood samples 
      were treated with heparin. The triple density gradient centrifugation, and MACS 
      using CD45 and CD71 were used to isolate the fetal cells. FISH analysis using 
      probe 21 was performed with GPA-immunostaining. The study population consisted of 
      30 patients from 13 to 25 weeks of gestation, and nRBCs were separated in all 
      cases. In GPA-immuno FISH analysis using probe 21, 3 cases of trisomy 21 were 
      diagnosed and these results were confirmed by the amniocentesis. In conclusion, a 
      prenatal diagnosis of trisomy 21 through GPA- immuno fluorescence in situ 
      hybridization (FISH) analysis using separated fetal nRBCs is a useful, 
      innovative, accurate, rapid and non-invasive diagnostic method.
FAU - Yang, Young Ho
AU  - Yang YH
AD  - Department of Obstetrics and Gynecology, Yonsei University College of Medicine, 
      134 Shinchon-dong, Seodaemun-gu, Seoul 120-752, Korea. ob@yumc.yonsei.ac.kr
FAU - Kim, Sung Hoon
AU  - Kim SH
FAU - Yang, Eun Suk
AU  - Yang ES
FAU - Kim, Sei Kwang
AU  - Kim SK
FAU - Kim, In Kyu
AU  - Kim IK
FAU - Park, Yong Won
AU  - Park YW
FAU - Cho, Jae Sung
AU  - Cho JS
FAU - Lee, Yoon Ho
AU  - Lee YH
LA  - eng
PT  - Journal Article
PL  - Korea (South)
TA  - Yonsei Med J
JT  - Yonsei medical journal
JID - 0414003
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Down Syndrome/*diagnosis
MH  - Female
MH  - Humans
MH  - Immunohistochemistry
MH  - In Situ Hybridization, Fluorescence
MH  - Pregnancy/*blood
MH  - Prenatal Diagnosis/*methods
EDAT- 2003/05/03 05:00
MHDA- 2003/05/30 05:00
CRDT- 2003/05/03 05:00
PHST- 2003/05/03 05:00 [pubmed]
PHST- 2003/05/30 05:00 [medline]
PHST- 2003/05/03 05:00 [entrez]
AID - 200304181 [pii]
AID - 10.3349/ymj.2003.44.2.181 [doi]
PST - ppublish
SO  - Yonsei Med J. 2003 Apr 30;44(2):181-6. doi: 10.3349/ymj.2003.44.2.181.