PMID- 12729905
OWN - NLM
STAT- MEDLINE
DCOM- 20030616
LR  - 20220331
IS  - 0014-5793 (Print)
IS  - 0014-5793 (Linking)
VI  - 542
IP  - 1-3
DP  - 2003 May 8
TI  - Redifferentiation of dedifferentiated bovine chondrocytes when cultured in vitro 
      in a PLGA-collagen hybrid mesh.
PG  - 95-9
AB  - Bovine articular chondrocytes dedifferentiated and lost their ability to express 
      articular cartilage-specific extracellular matrices such as type II collagen and 
      aggrecan when cultured in a culture flask during in vitro multiplication. A 
      poly(DL-lactic-co-glycolic acid) (PLGA)-collagen hybrid mesh was prepared and 
      used to redifferentiate the dedifferentiated cells. The two passaged 
      dedifferentiated chondrocytes were seeded in a PLGA-collagen hybrid mesh and 
      cultured in vitro in Dulbecco's modified Eagle's medium containing 10% fetal 
      bovine serum. The cells adhered to the hybrid mesh, distributed evenly, and 
      proliferated to fill the spaces in the scaffold. The gene expression of type I 
      collagen, type II collagen, and aggrecan was analyzed after the cells were 
      cultured in the hybrid mesh for 2-12 weeks. The expression of the gene encoding 
      type I collagen was downregulated, whereas those of type II collagen and aggrecan 
      were upregulated. Histological examination by hematoxylin-eosin and safranin 
      O/fast green staining indicates that the cells regained their original round 
      morphology. In addition, a homogeneous distribution of articular cartilage 
      extracellular matrices was detected around the cells. These results suggest 
      redifferentiation of the differentiated chondrocytes in the hybrid mesh. The 
      hybrid mesh, which facilitated the redifferentiation of the dedifferentiated 
      multiplied chondrocytes, would be an effective scaffold for the assembly of cells 
      to regenerate three-dimensional cartilaginous tissue.
FAU - Chen, Guoping
AU  - Chen G
AD  - Tissue Engineering Research Center, AIST Kansai, Amagasaki Site, National 
      Institute of Advanced Industrial Science and Technology, 3-11-46 Nakoji, 
      Amagasaki, Hyogo 661-0974, Japan. guoping-hen@aist.co.jp
FAU - Sato, Takashi
AU  - Sato T
FAU - Ushida, Takashi
AU  - Ushida T
FAU - Hirochika, Rei
AU  - Hirochika R
FAU - Tateishi, Tetsuya
AU  - Tateishi T
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - FEBS Lett
JT  - FEBS letters
JID - 0155157
RN  - 0 (Aggrecans)
RN  - 0 (Collagen Type I)
RN  - 0 (Collagen Type II)
RN  - 0 (Extracellular Matrix Proteins)
RN  - 0 (Lectins, C-Type)
RN  - 0 (Polymers)
RN  - 0 (Proteoglycans)
RN  - 0 (RNA, Messenger)
RN  - 1SIA8062RS (Polylactic Acid-Polyglycolic Acid Copolymer)
RN  - 26009-03-0 (Polyglycolic Acid)
RN  - 33X04XA5AT (Lactic Acid)
RN  - 9007-34-5 (Collagen)
SB  - IM
MH  - Aggrecans
MH  - Animals
MH  - Cattle
MH  - Cell Differentiation
MH  - Cells, Cultured
MH  - Chondrocytes/*cytology/drug effects/metabolism
MH  - Collagen/*pharmacology/ultrastructure
MH  - Collagen Type I/biosynthesis/genetics
MH  - Collagen Type II/biosynthesis/genetics
MH  - *Extracellular Matrix Proteins
MH  - Lactic Acid/chemistry/*pharmacology
MH  - Lectins, C-Type
MH  - Polyglycolic Acid/chemistry/*pharmacology
MH  - Polylactic Acid-Polyglycolic Acid Copolymer
MH  - Polymers/chemistry/*pharmacology
MH  - Proteoglycans/biosynthesis/genetics
MH  - RNA, Messenger/biosynthesis
MH  - *Tissue Engineering
EDAT- 2003/05/06 05:00
MHDA- 2003/06/17 05:00
CRDT- 2003/05/06 05:00
PHST- 2003/05/06 05:00 [pubmed]
PHST- 2003/06/17 05:00 [medline]
PHST- 2003/05/06 05:00 [entrez]
AID - S0014579303003545 [pii]
AID - 10.1016/s0014-5793(03)00354-5 [doi]
PST - ppublish
SO  - FEBS Lett. 2003 May 8;542(1-3):95-9. doi: 10.1016/s0014-5793(03)00354-5.