PMID- 12731856
OWN - NLM
STAT- MEDLINE
DCOM- 20030722
LR  - 20190714
IS  - 0013-936X (Print)
IS  - 0013-936X (Linking)
VI  - 37
IP  - 8
DP  - 2003 Apr 15
TI  - Bioreactors for removing methyl bromide following contained fumigations.
PG  - 1698-704
AB  - Use of methyl bromide (MeBr) as a quarantine, commodity, or structural fumigant 
      is under scrutiny because its release to the atmosphere contributes to the 
      depletion of stratospheric ozone. A closed-system bioreactor consisting of 0.5 L 
      of a growing culture of a previously described bacterium, strain IMB-1, removed 
      MeBr (> 110 micromol L(-1)) from recirculating air. Strain IMB-1 grew slowly to 
      high cell densities in the bioreactor using MeBr as its sole carbon and energy 
      source. Bacterial oxidation of MeBr produced CO2 and hydrobromic acid (HBr), 
      which required continuous neutralization with NaOH for the system to operate 
      effectively. Strain IMB-1 was capable of sustained oxidation of large amounts of 
      MeBr (170 mmol in 46 d). In an open-system bioreactor (10-L fermenter), strain 
      IMB-1 oxidized a continuous supply of MeBr (220 /micromol L(-1) in air). Growth 
      was continuous, and 0.5 mol of MeBr was removed from the air supply in 14 d. The 
      specific rate of MeBr oxidation was 7 x 10(-16) mol cell(-1) h(-1). Bioreactors 
      such as these can therefore be used to remove large quantities of contaminant 
      MeBr, which opens the possibility of biodegradation as a practical means for its 
      disposal.
FAU - Miller, Laurence G
AU  - Miller LG
AD  - U.S. Geological Survey, Mail Stop 465, 345 Middlefield Road, Menlo Park, 
      California 94025, USA. lgmiller@usgs.gov
FAU - Baesman, Shaun M
AU  - Baesman SM
FAU - Oremland, Ronald S
AU  - Oremland RS
LA  - eng
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - United States
TA  - Environ Sci Technol
JT  - Environmental science & technology
JID - 0213155
RN  - 0 (Hydrocarbons, Brominated)
RN  - 9V42E1Z7B6 (methyl bromide)
SB  - IM
MH  - Air Pollution/*prevention & control
MH  - Bacteria
MH  - Biodegradation, Environmental
MH  - *Bioreactors
MH  - Fumigation
MH  - Hydrocarbons, Brominated/chemistry/*isolation & purification
MH  - Oxidation-Reduction
EDAT- 2003/05/07 05:00
MHDA- 2003/07/23 05:00
CRDT- 2003/05/07 05:00
PHST- 2003/05/07 05:00 [pubmed]
PHST- 2003/07/23 05:00 [medline]
PHST- 2003/05/07 05:00 [entrez]
AID - 10.1021/es026155j [doi]
PST - ppublish
SO  - Environ Sci Technol. 2003 Apr 15;37(8):1698-704. doi: 10.1021/es026155j.