PMID- 12733117
OWN - NLM
STAT- MEDLINE
DCOM- 20030530
LR  - 20141120
IS  - 0046-8177 (Print)
IS  - 0046-8177 (Linking)
VI  - 34
IP  - 4
DP  - 2003 Apr
TI  - Evaluation of HER-2/neu oncogene status in breast tumors on tissue microarrays.
PG  - 362-8
AB  - The amplification and/or overexpression of the HER-2/neu oncogene and its encoded 
      receptor protein are increasingly used for prognostication and prediction of 
      therapeutic response to Herceptin in breast cancer. However, large-scale 
      examination of archival tumor blocks by immunohistochemistry (IHC) or 
      fluorescence in situ hybridization (FISH) is prohibitively laborious and 
      technically challenging. The tissue microarray (TMA) technique enables hundreds 
      of tumors to be studied simultaneously in a single experiment. To evaluate the 
      HER-2/neu status of a selection of the breast tumors in our tumor bank, we 
      constructed a TMA from 97 breast tumors, with a single 0.6-mm core per specimen. 
      HER-2/neu gene amplification by FISH was found in 20 of the 87 interpretable 
      cases (23%): in 14 of 14 IHC 3+ cases (100%), 5 of 8 IHC 2+ cases (62.5%) and 1 
      of 65 IHC 0/1+ cases (1.5%). Three of the 67 cases with no evidence of HER-2/neu 
      gene amplification by FISH were moderately positive (2+) by IHC. A close 
      relationship was observed between these 2 assays as applied to the TMA (95.4% 
      concordance: 95% CI, -2.2% to 6.8%; P <0.0001), and both HER-2/neu gene 
      amplification and protein overexpression were strongly associated with tumor 
      grade, estrogen receptor status, and progesterone receptor status. Gene 
      amplification was found in most of the tumors with high-level overexpression (IHC 
      3+) and not in the unequivocal IHC-negative cases. Complementary analysis by IHC 
      and FISH are, however, recommended for tumors graded as 2+ by IHC, the group with 
      the most result discrepancy. Hum Pathol 34:362-368.
CI  - Copyright 2003 Elsevier Inc. All rights reserved.
FAU - Zhang, Daohai
AU  - Zhang D
AD  - Molecular Diagnosis Centre, Department of Laboratory Medicine, National 
      University Hospital and Department of Pathology, National University of 
      Singapore, Singapore.
FAU - Salto-Tellez, Manuel
AU  - Salto-Tellez M
FAU - Do, Elaine
AU  - Do E
FAU - Putti, Thomas Choudary
AU  - Putti TC
FAU - Koay, Evelyn S C
AU  - Koay ES
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Hum Pathol
JT  - Human pathology
JID - 9421547
RN  - 0 (Reagent Kits, Diagnostic)
RN  - EC 2.7.10.1 (Receptor, ErbB-2)
SB  - IM
MH  - Breast Neoplasms/*genetics/metabolism
MH  - Female
MH  - *Gene Expression Profiling
MH  - Histocytological Preparation Techniques/*methods
MH  - Humans
MH  - Immunoenzyme Techniques
MH  - In Situ Hybridization, Fluorescence
MH  - *Oligonucleotide Array Sequence Analysis
MH  - Prognosis
MH  - Reagent Kits, Diagnostic
MH  - Receptor, ErbB-2/*genetics/metabolism
EDAT- 2003/05/07 05:00
MHDA- 2003/05/31 05:00
CRDT- 2003/05/07 05:00
PHST- 2003/05/07 05:00 [pubmed]
PHST- 2003/05/31 05:00 [medline]
PHST- 2003/05/07 05:00 [entrez]
AID - S0046817703000157 [pii]
AID - 10.1053/hupa.2003.60 [doi]
PST - ppublish
SO  - Hum Pathol. 2003 Apr;34(4):362-8. doi: 10.1053/hupa.2003.60.