PMID- 12734641
OWN - NLM
STAT- MEDLINE
DCOM- 20040129
LR  - 20191210
IS  - 0340-5761 (Print)
IS  - 0340-5761 (Linking)
VI  - 77
IP  - 5
DP  - 2003 May
TI  - Development of a high-performance reporter plasmid for detection of chemicals 
      with androgenic activity.
PG  - 274-9
AB  - A number of chemicals are present in the environment, and some synthetic 
      chemicals may disrupt endocrine function of wild animals and humans. An effective 
      procedure to screen chemicals for endocrine modulating activity has been needed 
      to ensure the safety of chemicals, and the reporter gene assay technique may 
      provide a powerful tool for screening endocrine-disrupting chemicals. We have 
      developed a high-performance reporter plasmid that can trigger high 
      androgen-dependent induction with high selectivity by using mouse mammary tumor 
      virus (MMTV) androgen-responsive elements and a partial fragment of the rat 
      alpha(2u)-globulin promoter region. This new type plasmid can induce higher 
      transcriptional activation than a commercial PGV-P-based construct bearing the 
      SV40 promoter fragment, and the basal induction level of this plasmid is much 
      lower than that of the PGV-P-based construct. Moreover, only androgen derivatives 
      could selectively induce a high response in the reporter gene assay with the new 
      reporter plasmid. This new type of reporter plasmid, ARE-AUG- Luc+, should be of 
      value in endocrine research and in screening to identify endocrine-modulating 
      chemicals.
FAU - Takeyoshi, Masahiro
AU  - Takeyoshi M
AD  - Chemicals Assessment Center, Chemicals Evaluation and Research Institute, 3-822 
      Ishii-machi, Hita-shi, 8770061, Oita, Japan. takeyoshi-masahiro@ceri.jp
FAU - Kuga, Naoko
AU  - Kuga N
FAU - Yamasaki, Kanji
AU  - Yamasaki K
LA  - eng
PT  - Journal Article
DEP - 20030220
PL  - Germany
TA  - Arch Toxicol
JT  - Archives of toxicology
JID - 0417615
RN  - 0 (Alpha-Globulins)
RN  - 0 (Androgen Antagonists)
RN  - 0 (Environmental Pollutants)
RN  - 0 (Indicators and Reagents)
RN  - 0 (alpha 2u globulin)
SB  - IM
MH  - Alpha-Globulins/genetics
MH  - Androgen Antagonists/classification/*toxicity
MH  - Animals
MH  - Chlorocebus aethiops
MH  - Dose-Response Relationship, Drug
MH  - Drug Evaluation, Preclinical/*methods
MH  - Environmental Pollutants/classification/*toxicity
MH  - *Genes, Reporter
MH  - Humans
MH  - Indicators and Reagents
MH  - Mammary Tumor Virus, Mouse/genetics
MH  - Plasmids/*genetics
MH  - Rats
EDAT- 2003/05/08 05:00
MHDA- 2004/01/30 05:00
CRDT- 2003/05/08 05:00
PHST- 2002/06/18 00:00 [received]
PHST- 2002/12/05 00:00 [accepted]
PHST- 2003/05/08 05:00 [pubmed]
PHST- 2004/01/30 05:00 [medline]
PHST- 2003/05/08 05:00 [entrez]
AID - 10.1007/s00204-003-0437-z [doi]
PST - ppublish
SO  - Arch Toxicol. 2003 May;77(5):274-9. doi: 10.1007/s00204-003-0437-z. Epub 2003 Feb 
      20.