PMID- 12735468
OWN - NLM
STAT- MEDLINE
DCOM- 20040128
LR  - 20190720
IS  - 0021-9673 (Print)
IS  - 0021-9673 (Linking)
VI  - 992
IP  - 1-2
DP  - 2003 Apr 11
TI  - Multiresidue method for fourteen fungicides in white grapes by liquid-liquid and 
      solid-phase extraction followed by liquid chromatography-diode array detection.
PG  - 121-31
AB  - A quantitative, selective and sensitive HPLC method for the analysis of 14 
      fungicides in white grapes for vinification is described. The proposed method is 
      based on liquid-liquid extraction (LLE) and solid-phase extraction (SPE) followed 
      by liquid chromatography and diode array detection (HPLC-DAD). 
      Dichloromethane-acetone (75:25, v/v) was the most appropriate solvent mix for 
      extracting fungicides in white grapes. Silica cartridges resulted the most 
      appropriate for extract purification purposes. Quality parameters of the proposed 
      multiresidue method presented good recovery (ca. 85% for almost all target 
      compounds) and precision (between 1.5 and 16%), and detection limits lower than 
      maxima residual limits set by the 76/895/ECC and 90/642/ECC Directive. Five 
      different white grapes for vinification produced in Rias Baixas area in Galicia 
      (NW Spain) were analyzed in order to assess the performance of the method with 
      real samples and to determine whether the concentration of the pesticides used 
      exceed their maxima residue levels (MRLs). Results showed that grape 
      concentrations for those identified fungicides were lower than those established 
      by European legislation.
FAU - Rial Otero, R
AU  - Rial Otero R
AD  - Nutrition and Bromatology Group, Analytical and Food Chemistry Department, 
      Faculty of Food Science and Technology, University of Vigo, Ourense Campus, 32004 
      Ourense, Spain.
FAU - Cancho Grande, B
AU  - Cancho Grande B
FAU - Simal Gandara, J
AU  - Simal Gandara J
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - J Chromatogr A
JT  - Journal of chromatography. A
JID - 9318488
RN  - 0 (Fungicides, Industrial)
SB  - IM
MH  - Chromatography, High Pressure Liquid/*methods
MH  - Fungicides, Industrial/*analysis
MH  - Reproducibility of Results
MH  - Sensitivity and Specificity
MH  - Spectrophotometry, Ultraviolet/*methods
MH  - Vitis/*chemistry
EDAT- 2003/05/09 05:00
MHDA- 2004/01/30 05:00
CRDT- 2003/05/09 05:00
PHST- 2003/05/09 05:00 [pubmed]
PHST- 2004/01/30 05:00 [medline]
PHST- 2003/05/09 05:00 [entrez]
AID - S0021-9673(03)00317-0 [pii]
AID - 10.1016/s0021-9673(03)00317-0 [doi]
PST - ppublish
SO  - J Chromatogr A. 2003 Apr 11;992(1-2):121-31. doi: 10.1016/s0021-9673(03)00317-0.