PMID- 12736140
OWN - NLM
STAT- MEDLINE
DCOM- 20030925
LR  - 20200930
IS  - 0363-6143 (Print)
IS  - 0363-6143 (Linking)
VI  - 285
IP  - 3
DP  - 2003 Sep
TI  - Acridine orange induces translocation of phosphatidylserine to red blood cell 
      surface.
PG  - C720-2
AB  - Clustering of band-3 on red blood cell (RBC) surface has been assumed to catalyze 
      RBC phagocytosis. In studying this subject, acridine orange (AO) has commonly 
      been employed on the assumption that it specifically induces band-3 clustering. 
      In the present study, we show that AO strongly induces translocation of 
      phosphatidylserine (PS) to RBC surface. Because surface PS is well known to 
      induce RBC intercellular interaction, these findings suggest that the use of AO 
      as a specific inducer of band-3 clustering is questionable. It is possible that 
      band-3 clustering and PS translocation are interdependent, and this 
      interrelationship has yet to be explored.
FAU - Koshkaryev, Alexander
AU  - Koshkaryev A
AD  - Department of Biochemistry, Hebrew University-Hadassah Medical School, Jerusalem, 
      Israel 91120.
FAU - Yedgar, Saul
AU  - Yedgar S
FAU - Relevy, Hanna
AU  - Relevy H
FAU - Fibach, Eithan
AU  - Fibach E
FAU - Barshtein, Gregory
AU  - Barshtein G
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20030507
PL  - United States
TA  - Am J Physiol Cell Physiol
JT  - American journal of physiology. Cell physiology
JID - 100901225
RN  - 0 (Anion Exchange Protein 1, Erythrocyte)
RN  - 0 (Mutagens)
RN  - 0 (Phosphatidylserines)
RN  - F30N4O6XVV (Acridine Orange)
SB  - IM
MH  - Acridine Orange/*pharmacology
MH  - Anion Exchange Protein 1, Erythrocyte/metabolism
MH  - Cell Adhesion/drug effects
MH  - Erythrocytes/*drug effects/*metabolism
MH  - Humans
MH  - Mutagens/*pharmacology
MH  - Phosphatidylserines/*metabolism
EDAT- 2003/05/09 05:00
MHDA- 2003/09/26 05:00
CRDT- 2003/05/09 05:00
PHST- 2003/05/09 05:00 [pubmed]
PHST- 2003/09/26 05:00 [medline]
PHST- 2003/05/09 05:00 [entrez]
AID - 00542.2002 [pii]
AID - 10.1152/ajpcell.00542.2002 [doi]
PST - ppublish
SO  - Am J Physiol Cell Physiol. 2003 Sep;285(3):C720-2. doi: 
      10.1152/ajpcell.00542.2002. Epub 2003 May 7.