PMID- 12736347
OWN - NLM
STAT- MEDLINE
DCOM- 20030619
LR  - 20200225
IS  - 1529-2401 (Electronic)
IS  - 0270-6474 (Print)
IS  - 0270-6474 (Linking)
VI  - 23
IP  - 9
DP  - 2003 May 1
TI  - Long-term depression is not induced by low-frequency stimulation in rat visual 
      cortex in vivo: a possible preventing role of endogenous brain-derived 
      neurotrophic factor.
PG  - 3761-70
AB  - Low-frequency stimulation (LFS) at 1 Hz for 15 min is an effective protocol to 
      induce homosynaptic long-term depression (LTD) in visual cortical slices. It is 
      reported that LFS becomes ineffective when brain-derived neurotrophic factor 
      (BDNF) is applied to slices. It is not known, however, whether such a protocol 
      induces LTD in visual cortex in vivo, and whether endogenous BDNF has the same or 
      similar action. To address these questions, we recorded field potentials of rat 
      visual cortex evoked by stimulation of lateral geniculate nucleus, white matter, 
      or cortical layer IV. We found that LFS did not induce LTD of cortical responses 
      in vivo. To test the possibility that spontaneous activity from retinas would 
      interfere with the induction of LTD, both eyes were removed or inactivated by 
      tetrodotoxin. LTD was not induced in these conditions either. To test whether the 
      difference in temperature between the two preparations is a factor for the 
      discrepancy, the temperature of slices was increased from 31 to 37 degrees C. LTD 
      was induced in slices at either temperature. Then, we hypothesized that 
      endogenous BNDF and its receptors, TrkB, prevent the induction of LTD. To test 
      this, we infused the cortex with an inhibitor of Trk receptor tyrosine kinases, 
      anti-TrkB IgG1, anti-BDNF, and anti-neurotrophin 4/5 antibodies. LTD was induced 
      when the BDNF-TrkB system was blocked. In slices, the level of phosphorylation of 
      Trks was found to decrease with time. These results indicate that activation of 
      TrkB signal pathway prevents LFS from inducing synaptic depression in visual 
      cortex in vivo.
FAU - Jiang, Bin
AU  - Jiang B
AD  - Core Research for Evolutional Science and Technology, Japan Science and 
      Technology Corporation, Kawaguchi, Saitama 332-0012 Japan.
FAU - Akaneya, Yukio
AU  - Akaneya Y
FAU - Hata, Yoshio
AU  - Hata Y
FAU - Tsumoto, Tadaharu
AU  - Tsumoto T
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Neurosci
JT  - The Journal of neuroscience : the official journal of the Society for 
      Neuroscience
JID - 8102140
RN  - 0 (Antibodies)
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Enzyme Inhibitors)
RN  - 0 (Nerve Growth Factors)
RN  - 145172-44-7 (neurotrophin 5)
RN  - 4368-28-9 (Tetrodotoxin)
RN  - EC 2.7.10.1 (Receptor, trkB)
RN  - P658DCA9XD (neurotrophin 4)
SB  - IM
MH  - Animals
MH  - Antibodies/pharmacology
MH  - Brain-Derived Neurotrophic Factor/antagonists & inhibitors/*physiology
MH  - Diffusion
MH  - Electric Stimulation/methods
MH  - Enzyme Inhibitors/pharmacology
MH  - Geniculate Bodies/physiology
MH  - In Vitro Techniques
MH  - Long-Term Synaptic Depression/drug effects/*physiology
MH  - Membrane Potentials/physiology
MH  - Nerve Growth Factors/antagonists & inhibitors
MH  - Phosphorylation/drug effects
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Receptor, trkB/antagonists & inhibitors
MH  - Retina/drug effects/physiology
MH  - Signal Transduction/drug effects/physiology
MH  - Temperature
MH  - Tetrodotoxin/pharmacology
MH  - Visual Cortex/cytology/drug effects/metabolism/*physiology
PMC - PMC6742196
EDAT- 2003/05/09 05:00
MHDA- 2003/06/20 05:00
PMCR- 2003/11/01
CRDT- 2003/05/09 05:00
PHST- 2003/05/09 05:00 [pubmed]
PHST- 2003/06/20 05:00 [medline]
PHST- 2003/05/09 05:00 [entrez]
PHST- 2003/11/01 00:00 [pmc-release]
AID - 23/9/3761 [pii]
AID - 7573 [pii]
AID - 10.1523/JNEUROSCI.23-09-03761.2003 [doi]
PST - ppublish
SO  - J Neurosci. 2003 May 1;23(9):3761-70. doi: 10.1523/JNEUROSCI.23-09-03761.2003.