PMID- 12738467
OWN - NLM
STAT- MEDLINE
DCOM- 20030611
LR  - 20081121
IS  - 0002-9270 (Print)
IS  - 0002-9270 (Linking)
VI  - 98
IP  - 4
DP  - 2003 Apr
TI  - Cytokine genotyping (TNF and IL-10) in patients with celiac disease and selective 
      IgA deficiency.
PG  - 850-6
AB  - OBJECTIVE: Selective IgA deficiency (IgAD) and celiac disease (CD) are frequently 
      associated and share the ancestral haplotype human leukocyte antigen (HLA)-8.1, 
      which is characterized by a peculiar cytokine profile. The aim of this study was 
      to evaluate the role of tumor necrosis factor (TNF) and interleukin (IL)-10 
      alleles in CD and CD-IgAD. METHODS: The distribution of some biallelic 
      polymorphisms of both cytokine promoters (-308G-->A and -863C-->A at TNF promoter 
      sequence and -1082G-->A, -819C-->A, and -592C-->T at IL-10 promoter) were typed 
      using biotilinated specific probes in 32 celiac patients, in 34 CD-IgAD patients, 
      and in 96 healthy controls. RESULTS: In CD and CD-IgAD, the -308A allele was 
      significantly more frequent than in controls, whereas no significant differences 
      were observed for the biallelic polymorphisms at the -863 and for the three IL-10 
      promoter polymorphisms. The evaluation of combined TNF and IL-10 genotypes showed 
      in CD-IgAD a significant reduction of -308G/-1082G homozygous subjects and both 
      in CD and CD-IgAD groups an increase of 308AA/1082GG. Accordingly, CD-IgAD 
      patients positive both for -308A TNF and -1082A IL-10 showed an increase of 
      TNF-alpha and a reduction of IL-10 serum levels. CONCLUSIONS: Genetically 
      determined increased production of TNF-alpha and reduction of IL-10 may be 
      relevant for susceptibility to CD, mainly in IgAD, as the different allele 
      expression at TNF and IL-10 loci seems to influence cytokine production profile.
FAU - Cataldo, F
AU  - Cataldo F
AD  - Clinica Pediatrica R, Sezione di Patologia Generale, University of Palermo, 
      Palermo, Italy.
FAU - Lio, D
AU  - Lio D
FAU - Marino, V
AU  - Marino V
FAU - Scola, L
AU  - Scola L
FAU - Crivello, A
AU  - Crivello A
FAU - Mule, A M
AU  - Mule AM
FAU - Corazza, G R
AU  - Corazza GR
CN  - Groups of the SIGEP and "Club del Tenue"
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Am J Gastroenterol
JT  - The American journal of gastroenterology
JID - 0421030
RN  - 0 (Cytokines)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 130068-27-8 (Interleukin-10)
SB  - IM
MH  - Adult
MH  - Celiac Disease/blood/complications/*genetics
MH  - Child
MH  - Cross-Sectional Studies
MH  - Cytokines/*genetics
MH  - Gene Frequency/genetics
MH  - *Genotype
MH  - Humans
MH  - IgA Deficiency/blood/complications/*genetics
MH  - Interleukin-10/blood/*genetics
MH  - Polymorphism, Genetic/genetics
MH  - Promoter Regions, Genetic/genetics
MH  - Sequence Analysis
MH  - Tumor Necrosis Factor-alpha/analysis/*genetics
EDAT- 2003/05/10 05:00
MHDA- 2003/06/12 05:00
CRDT- 2003/05/10 05:00
PHST- 2003/05/10 05:00 [pubmed]
PHST- 2003/06/12 05:00 [medline]
PHST- 2003/05/10 05:00 [entrez]
AID - S0002927003000418 [pii]
AID - 10.1111/j.1572-0241.2003.t01-1-07377.x [doi]
PST - ppublish
SO  - Am J Gastroenterol. 2003 Apr;98(4):850-6. doi: 
      10.1111/j.1572-0241.2003.t01-1-07377.x.