PMID- 12738673
OWN - NLM
STAT- MEDLINE
DCOM- 20031002
LR  - 20210206
IS  - 0006-4971 (Print)
IS  - 0006-4971 (Linking)
VI  - 102
IP  - 5
DP  - 2003 Sep 1
TI  - Functional comparison of DCs generated in vivo with Flt3 ligand or in vitro from 
      blood monocytes: differential regulation of function by specific classes of 
      physiologic stimuli.
PG  - 1753-63
AB  - Dendritic cells (DCs) are a family of leukocytes that initiate T- and B-cell 
      immunity against pathogens. Migration of antigen-loaded DCs from sites of 
      infection into draining lymphoid tissues is fundamental to the priming of T-cell 
      immune responses. In humans, the major peripheral blood DC (PBDC) types, CD1c+ 
      DCs and interleukin 3 receptor-positive (IL-3R+) plasmacytoid DCs, are 
      significantly expanded in vivo with the use of Flt3 ligand (FL). DC-like cells 
      can also be generated from monocyte precursors (MoDCs). A detailed comparison of 
      the functional potential of these types of DCs (in an autologous setting) has yet 
      to be reported. Here, we compared the functional capacity of FL-expanded CD1c+ 
      PBDCs with autologous MoDCs in response to 3 different classes of stimuli: (1) 
      proinflammatory mediators, (2) soluble CD40 ligand trimer (CD40L), and (3) intact 
      bacteria (Escherichia coli). Significant differences in functional capacities 
      were found with respect to changes in phenotype, migratory capacity, cytokine 
      secretion, and T-cell stimulation. MoDCs required specific stimuli for the 
      expression of functions. They responded vigorously to CD40L or E coli, expressing 
      cytokines known to regulate interferon-gamma (IFN-gamma) in T cells (IL-12p70, 
      IL-18, and IL-23), but required prostaglandin E2 (PGE2) during stimulation to 
      migrate to chemokines. In contrast, PBDCs matured in response to minimal 
      stimulation, rapidly acquired migratory function in the absence of 
      PGE2-containing stimuli, and were low cytokine producers. Interestingly, both 
      types of DCs were equivalent with respect to stimulation of allogeneic T-cell 
      proliferation and presentation of peptides to cytotoxic T lymphocyte (CTL) lines. 
      These distinct differences are of particular importance when considering the 
      choice of DC types for clinical applications.
FAU - Jefford, Michael
AU  - Jefford M
AD  - Ludwig Institute Oncology Unit, Austin and Repatriation Medical Centre, Studley 
      Rd, Heidelberg, Victoria 3084, Australia.
FAU - Schnurr, Max
AU  - Schnurr M
FAU - Toy, Tracey
AU  - Toy T
FAU - Masterman, Kelly-Anne
AU  - Masterman KA
FAU - Shin, Amanda
AU  - Shin A
FAU - Beecroft, Tina
AU  - Beecroft T
FAU - Tai, Tsin Yee
AU  - Tai TY
FAU - Shortman, Ken
AU  - Shortman K
FAU - Shackleton, Mark
AU  - Shackleton M
FAU - Davis, Ian D
AU  - Davis ID
FAU - Parente, Phil
AU  - Parente P
FAU - Luft, Thomas
AU  - Luft T
FAU - Chen, Weisan
AU  - Chen W
FAU - Cebon, Jonathan
AU  - Cebon J
FAU - Maraskovsky, Eugene
AU  - Maraskovsky E
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20030508
PL  - United States
TA  - Blood
JT  - Blood
JID - 7603509
RN  - 0 (Antigens, CD1)
RN  - 0 (CD1C protein, human)
RN  - 0 (Cytokines)
RN  - 0 (Glycoproteins)
RN  - 0 (Inflammation Mediators)
RN  - 0 (Membrane Proteins)
RN  - 0 (Peptides)
RN  - 0 (flt3 ligand protein)
RN  - 147205-72-9 (CD40 Ligand)
SB  - IM
MH  - Antigens, CD1/metabolism
MH  - CD40 Ligand/pharmacology
MH  - Cell Differentiation/drug effects/immunology
MH  - Cell Division/immunology
MH  - Cell Movement/immunology
MH  - Cells, Cultured
MH  - Cytokines/biosynthesis
MH  - Dendritic Cells/*cytology/*immunology/metabolism
MH  - Escherichia coli
MH  - Glycoproteins/metabolism
MH  - Humans
MH  - Immunophenotyping
MH  - In Vitro Techniques
MH  - Inflammation Mediators/pharmacology
MH  - Lymphocyte Activation/immunology
MH  - Melanoma/immunology
MH  - Membrane Proteins/*pharmacology
MH  - Monocytes/*cytology
MH  - Peptides/pharmacology
MH  - Stimulation, Chemical
MH  - T-Lymphocytes/cytology/immunology
EDAT- 2003/05/10 05:00
MHDA- 2003/10/03 05:00
CRDT- 2003/05/10 05:00
PHST- 2003/05/10 05:00 [pubmed]
PHST- 2003/10/03 05:00 [medline]
PHST- 2003/05/10 05:00 [entrez]
AID - S0006-4971(20)44162-X [pii]
AID - 10.1182/blood-2002-12-3854 [doi]
PST - ppublish
SO  - Blood. 2003 Sep 1;102(5):1753-63. doi: 10.1182/blood-2002-12-3854. Epub 2003 May 
      8.