PMID- 12739032
OWN - NLM
STAT- MEDLINE
DCOM- 20040504
LR  - 20181113
IS  - 0937-4477 (Print)
IS  - 0937-4477 (Linking)
VI  - 260
IP  - 9
DP  - 2003 Oct
TI  - Expression of collagenase-1 (MMP-1), collagenase-3 (MMP-13) and tissue inhibitor 
      of matrix metalloproteinase-1 (TIMP-1) in laryngeal squamous cell carcinomas.
PG  - 494-7
AB  - Matrix metalloproteinases (MMPs) that have a proteolytic activity against the 
      components of extracellular matrix (ECM) play an important role in the invasive 
      and metastatic spread of tumors. The role of MMPs and tissue inhibitors of MMPs 
      (TIMPs) in laryngeal squamous cell carcinoma (LSCC) has not been elucidated 
      sufficiently. The aim of the present study was to evaluate the correlation 
      between the expression of collagenase-1 (MMP-1), collagenase-3 (MMP-13) and 
      TIMP-1, as well as the clinicopathological features of LSCCs. The expression of 
      collagenases and TIMP-1 was examined immunohistochemically in 50 cases of 
      surgically obtained specimens of primary LSCCs. Analyses indicated that LSCC 
      cells as well as stromal cells expressed MMP-1, MMP-13 and TIMP-1 immunostaining. 
      Overexpression of TIMP-1 occurred more frequently in non-metastasizing cases 
      (P=0.009). TIMP-1 and MMP-1 staining correlated significantly with the histologic 
      type of LSCC. The keratinizing type of carcinomas exhibited higher TIMP-1 protein 
      expression than the nonkeratinizing variety (P=0.01). TIMP-1 staining was 
      associated with the grade of differentiation, since it was found predominantly in 
      well and moderately differentiated carcinomas (P=0.04). The findings confirm that 
      expression of analyzed MMPs and TIMP-1 is characteristic of LSCC and that these 
      enzymes contribute to the progression of tumors. TIMP-1 upregulation might 
      exhibit lower metastatic potential in LSCCs and is linked rather with an early 
      stage of tumor progression. It seems also that TIMP-1 expression is dependent on 
      the grade of differentiation.
FAU - Krecicki, Tomasz
AU  - Krecicki T
AD  - Department of Otolaryngology, Wroclaw Medical University, Chalubinskiego Street 
      2, 51-268 Wroclaw, Poland. krecicki@orl.am.wroc.pl
FAU - Fraczek, Marcin
AU  - Fraczek M
FAU - Jelen, Michal
AU  - Jelen M
FAU - Podhorska, Marzena
AU  - Podhorska M
FAU - Szkudlarek, Teresa
AU  - Szkudlarek T
FAU - Zatonski, Tomasz
AU  - Zatonski T
LA  - eng
PT  - Journal Article
DEP - 20030509
PL  - Germany
TA  - Eur Arch Otorhinolaryngol
JT  - European archives of oto-rhino-laryngology : official journal of the European 
      Federation of Oto-Rhino-Laryngological Societies (EUFOS) : affiliated with the 
      German Society for Oto-Rhino-Laryngology - Head and Neck Surgery
JID - 9002937
RN  - 0 (Tissue Inhibitor of Metalloproteinase-1)
RN  - 9007-34-5 (Collagen)
RN  - EC 3.4.24.- (Collagenases)
RN  - EC 3.4.24.- (MMP13 protein, human)
RN  - EC 3.4.24.- (Matrix Metalloproteinase 13)
RN  - EC 3.4.24.- (collagenase 1)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Carcinoma, Squamous Cell/*enzymology/genetics/metabolism
MH  - Collagen/metabolism
MH  - Collagenases/genetics/*metabolism
MH  - Female
MH  - Gene Expression Regulation, Enzymologic
MH  - Gene Expression Regulation, Neoplastic
MH  - Humans
MH  - Immunohistochemistry
MH  - Laryngeal Neoplasms/*enzymology/genetics/metabolism
MH  - Male
MH  - Matrix Metalloproteinase 13
MH  - Middle Aged
MH  - Tissue Inhibitor of Metalloproteinase-1/genetics/*metabolism
EDAT- 2003/05/10 05:00
MHDA- 2004/05/05 05:00
CRDT- 2003/05/10 05:00
PHST- 2002/10/21 00:00 [received]
PHST- 2003/03/26 00:00 [accepted]
PHST- 2003/05/10 05:00 [pubmed]
PHST- 2004/05/05 05:00 [medline]
PHST- 2003/05/10 05:00 [entrez]
AID - 10.1007/s00405-003-0610-2 [doi]
PST - ppublish
SO  - Eur Arch Otorhinolaryngol. 2003 Oct;260(9):494-7. doi: 10.1007/s00405-003-0610-2. 
      Epub 2003 May 9.