PMID- 12742156
OWN - NLM
STAT- MEDLINE
DCOM- 20030610
LR  - 20190816
IS  - 0165-4608 (Print)
IS  - 0165-4608 (Linking)
VI  - 143
IP  - 1
DP  - 2003 May
TI  - Genomic rearrangements involving rDNA and centromeric heterochromatin in vulvar 
      epidermoid carcinoma cell line A-431.
PG  - 50-8
AB  - The cytogenetic and molecular cytogenetic characterization of the human cell line 
      A-431 derived from a vulvar epidermoid carcinoma is presented. A combination of 
      karyotyping, fluorescence in situ hybridization (FISH) with chromosome- and/or 
      region-specific probes, M-FISH, RxFISH, and comparative genomic hybridization 
      (CGH) analysis was used. Six marker chromosomes with rearrangements involving 
      insertions of single or double nucleolar organizing regions (NORs) and/or 
      homogeneously staining regions containing active and overexpressed NORs and 
      regions of centromeric heterochromatin were found: der(6), der(7), der(17), 
      der(21), dic(13;14), and dic(14;18). The chromosomal origin of 14 other marker 
      chromosomes was elucidated. Amplification of the C-MYC oncogene at 8q24 was 
      revealed in two marker chromosomes: dup(8)(q24) and der(15)t(8;15)(q22;p11). 
      Confirming previous reports, amplification of the cyclin D1 gene within an 
      abnormal chromosome 11, that is, der(11)t(7;11)(p15;q21), was also detected. Loss 
      of the TP53 tumor suppressor gene was evidenced over two der(17). Good 
      concordance was found among karyotyping, FISH analysis, and CGH. Although reasons 
      for NOR amplification or ectopic location in the epidermal carcinoma A-431 cell 
      line are not clear yet, our data suggest that these phenomena play a supporting 
      role with regard to other amplified genes. Thus, the A-431 cell line would be an 
      appropriate model to study the different mechanisms involved in human 
      tumorigenesis.
FAU - Pedrazzini, Estela
AU  - Pedrazzini E
AD  - Departamento de Genetica, Instituto de Investigaciones Hematologicas "Mariano R. 
      Castex," Academia Nacional de Medicina, Pacheco de Melo 3081, Buenos Aires 1425, 
      Argentina. epedrazzini@hotmail.com
FAU - Mamaev, Nikolai
AU  - Mamaev N
FAU - Yakovleva, Tatyana
AU  - Yakovleva T
FAU - Sukhikh, Tatyana
AU  - Sukhikh T
FAU - Salido, Marta
AU  - Salido M
FAU - Sole, Francesc
AU  - Sole F
FAU - Prat, Esther
AU  - Prat E
FAU - Camps, Jordi
AU  - Camps J
FAU - Miro, Rosa
AU  - Miro R
FAU - Slavutsky, Irma
AU  - Slavutsky I
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Cancer Genet Cytogenet
JT  - Cancer genetics and cytogenetics
JID - 7909240
RN  - 0 (Genetic Markers)
SB  - IM
MH  - Carcinoma, Squamous Cell/*genetics
MH  - *Chromosome Aberrations
MH  - Female
MH  - Genetic Markers
MH  - Humans
MH  - Karyotyping
MH  - Tumor Cells, Cultured
MH  - Vulvar Neoplasms/*genetics
EDAT- 2003/05/14 05:00
MHDA- 2003/06/11 05:00
CRDT- 2003/05/14 05:00
PHST- 2003/05/14 05:00 [pubmed]
PHST- 2003/06/11 05:00 [medline]
PHST- 2003/05/14 05:00 [entrez]
AID - S0165460802008452 [pii]
AID - 10.1016/s0165-4608(02)00845-2 [doi]
PST - ppublish
SO  - Cancer Genet Cytogenet. 2003 May;143(1):50-8. doi: 10.1016/s0165-4608(02)00845-2.