PMID- 12742355
OWN - NLM
STAT- MEDLINE
DCOM- 20030623
LR  - 20190816
IS  - 0014-4835 (Print)
IS  - 0014-4835 (Linking)
VI  - 76
IP  - 6
DP  - 2003 Jun
TI  - Glycero- versus sphingo-phospholipids: correlations with human and non-human 
      mammalian lens growth.
PG  - 725-34
AB  - The human lens differs from other mammalian lenses in its very slow growth and 
      unusual phospholipid composition of its cell membranes. Dihydrosphingomyelins 
      (DHSMs) make up about half of all phospholipids in adult human fiber membranes. 
      In all other membranes, sphingomyelins(SMs) with a trans double bond in their 
      backbone, are prevalent. In our quest to understand the biological implications 
      of such elevated DHSM levels, we analyzed membranes from various regions of 
      human, elephant, giraffe, polar bear, pig and cow lenses. The levels of DHSMs 
      were minor in non-human lens membranes. A strong correlation was observed between 
      growth rate and relative contents of phosphatidylcholines(PCs) in epithelia and 
      outer cortical fibers. Sphingomyelins became increasingly predominant in 
      differentiated fibers and this increase was age dependent. Indeed, nuclear fiber 
      membranes of aged non-human mammals were composed, almost exclusively, of (SMs). 
      Although human lens membranes followed comparable compositional trends, the 
      magnitude of the changes was much smaller. We postulate that the high relative 
      contents of DHSMs provide a biochemically inert matrix in which only small 
      amounts of PCs and SMs and their metabolites, known to promote and arrest growth, 
      respectively, are present. This compositional difference is proposed to 
      contribute to the slow multiplication and elongation of human lens cells.
FAU - Yappert, M Cecilia
AU  - Yappert MC
AD  - Department of Chemistry, College of Arts and Sciences, University of Louisville, 
      Louisville, KY 40292, USA. mcyapper@louisville.edu
FAU - Rujoi, Madalina
AU  - Rujoi M
FAU - Borchman, Douglas
AU  - Borchman D
FAU - Vorobyov, Igor
AU  - Vorobyov I
FAU - Estrada, Rosendo
AU  - Estrada R
LA  - eng
GR  - EY11657/EY/NEI NIH HHS/United States
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - England
TA  - Exp Eye Res
JT  - Experimental eye research
JID - 0370707
RN  - 0 (Phosphatidylcholines)
RN  - 0 (Sphingomyelins)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Aged
MH  - Animals
MH  - Artiodactyla
MH  - Cattle
MH  - Elephants
MH  - Humans
MH  - Lens, Crystalline/chemistry/cytology/*physiology
MH  - Magnetic Resonance Spectroscopy
MH  - Mammals/*physiology
MH  - Phosphatidylcholines/analysis/metabolism
MH  - Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
MH  - Sphingomyelins/*analysis/metabolism
MH  - Swine
MH  - Ursidae
EDAT- 2003/05/14 05:00
MHDA- 2003/06/24 05:00
CRDT- 2003/05/14 05:00
PHST- 2003/05/14 05:00 [pubmed]
PHST- 2003/06/24 05:00 [medline]
PHST- 2003/05/14 05:00 [entrez]
AID - S0014483503000514 [pii]
AID - 10.1016/s0014-4835(03)00051-4 [doi]
PST - ppublish
SO  - Exp Eye Res. 2003 Jun;76(6):725-34. doi: 10.1016/s0014-4835(03)00051-4.