PMID- 12742510
OWN - NLM
STAT- MEDLINE
DCOM- 20030711
LR  - 20190702
IS  - 0027-5107 (Print)
IS  - 0027-5107 (Linking)
VI  - 537
IP  - 1
DP  - 2003 May 9
TI  - Exposure to acrylonitrile induced DNA strand breakage and sex chromosome 
      aneuploidy in human spermatozoa.
PG  - 93-100
AB  - To explore acrylonitrile (ACN)-induced DNA strand breakage and sex chromosome 
      aneuploidy in human spermatozoa, semen parameters were examined among 30 
      acrylonitrile-exposed workers according to WHO laboratory manual for the 
      examination of human sperm. DNA strand breakage of sperm cells was investigated 
      among 30 ACN-exposed workers using single cell gel electrophoresis (SCGE). The 
      frequency of sex chromosome aneuploidy in sperm cells was analyzed among nine 
      ACN-exposed workers using fluorescence in situ hybridization (FISH). The 
      geometrical mean of sperm density was 75 x 10(6)ml(-1) in exposure group, 
      significantly lower than 140 x 10(6)ml(-1) in the control. The geometrical mean 
      of sperm number per ejaculum was 205 x 10(6) in exposure group, significantly 
      lower than 280 x 10(6) in the control. The rates of comet sperm nuclei were 28.7% 
      in exposure group, significantly higher than 15.0% in the control. Mean tail 
      length was 9.8 microm in exposure group, longer than 4.3 microm in the control. 
      The frequency of sex chromosome disomy was 0.69% in exposure group, significantly 
      higher than 0.35% in the control. XY-bearing sperm was the most common sex 
      chromosome disomy, with an average rate of 0.37% in exposure group, and 0.20% in 
      the control. XX- and YY-bearing sperm accounted for an additional 0.09 and 0.23% 
      in exposure group, and 0.05 and 0.10% in the control. The results indicate that 
      ACN affect semen quality among ACN-exposed workers. ACN or its metabolites could 
      induce reproductive defects as an in vivo multipotent genotoxic agent by inducing 
      DNA strand breakage and sex chromosome non-disjunction in spermatogenesis.
FAU - Xu, De-Xiang
AU  - Xu DX
AD  - Department of Toxicology, School of Public Health, Anhui Medical University, 
      Hefei, PR China. xudex@mail.hf.ah.cn
FAU - Zhu, Qi-Xing
AU  - Zhu QX
FAU - Zheng, Lu-Kang
AU  - Zheng LK
FAU - Wang, Qu-Nan
AU  - Wang QN
FAU - Shen, Han-Ming
AU  - Shen HM
FAU - Deng, Li-Xia
AU  - Deng LX
FAU - Ong, Choon-Nam
AU  - Ong CN
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Mutat Res
JT  - Mutation research
JID - 0400763
RN  - MP1U0D42PE (Acrylonitrile)
SB  - IM
MH  - *Acrylonitrile
MH  - Adult
MH  - *Aneuploidy
MH  - Chromosomes/ultrastructure
MH  - *Chromosomes, Human, X
MH  - *Chromosomes, Human, Y
MH  - Comet Assay
MH  - *DNA Damage
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Male
MH  - Spermatozoa/*drug effects/*metabolism
EDAT- 2003/05/14 05:00
MHDA- 2003/07/12 05:00
CRDT- 2003/05/14 05:00
PHST- 2003/05/14 05:00 [pubmed]
PHST- 2003/07/12 05:00 [medline]
PHST- 2003/05/14 05:00 [entrez]
AID - S138357180300055X [pii]
AID - 10.1016/s1383-5718(03)00055-x [doi]
PST - ppublish
SO  - Mutat Res. 2003 May 9;537(1):93-100. doi: 10.1016/s1383-5718(03)00055-x.