PMID- 12743324
OWN - NLM
STAT- MEDLINE
DCOM- 20040227
LR  - 20171116
IS  - 1066-5099 (Print)
IS  - 1066-5099 (Linking)
VI  - 21
IP  - 3
DP  - 2003
TI  - Absolute values of dendritic cell subsets in bone marrow, cord blood, and 
      peripheral blood enumerated by a novel method.
PG  - 296-303
AB  - Dendritic cells (DCs) are pivotal in inducing immunity or alternatively 
      downregulating immune reactivity. In humans, the opposing phenotypic subsets of 
      CD11c(+)/CD123(-) "myeloid" DCs and CD123(+)/CD11c(-) "lymphoid" DCs have been 
      proposed to orchestrate these immune responses. In this study we determined the 
      absolute numbers of both subsets in three resting hematopoietic tissues by 
      employing a novel flow cytometry method, eliminating processing steps and 
      calculations based on mononuclear cell percentages. Internal bead standards along 
      with the cells of interest were simultaneously acquired directly from 
      unmanipulated whole blood specimens. We found significant differences (p < 0.001) 
      between the mean absolute numbers of CD123(+)/CD11c(-) lymphoid DCs among the 
      three sources, with the fewest present in peripheral blood (8.2/ micro l), about 
      50% more in cord blood (12.2/ micro l), and fivefold more in bone marrow (40.2/ 
      micro l). Cord blood and bone marrow CD11c(+)/CD123(-) myeloid DC counts did not 
      differ from each other (23.5/ micro l and 28.9/ micro l, respectively) but 
      peripheral blood contained significantly fewer (15.5/ micro l, p = 0.006). 
      CD11c(+)/CD123(-) DCs had significantly higher surface expression of HLA-DR (p < 
      0.001) in all three sources. To test for association with the DC subsets, T, B, 
      and natural killer (NK) lymphocytes were also enumerated. In bone marrow only, 
      significant correlations were found between the size of the CD123(+)/CD11c(-) 
      lymphoid DC pool and NK cells (p = 0.0029) and B cells (p = 0.0033). These data 
      support the hypothesis that a common CD123(+)/CD11c(-) DC, NK cell, and B cell 
      progenitor is resident in marrow, and this cell may be identical to the common 
      lymphoid progenitor previously described in mice and/or the human 
      CD34(+)/Lin(-)/CD10(+) progenitor.
FAU - Szabolcs, Paul
AU  - Szabolcs P
AD  - Department of Pediatrics, Pediatric Stem Cell Transplant Program, Duke University 
      Medical Center, Durham, North Carolina 27710, USA. szabo001@mc.duke.edu
FAU - Park, Kyung-Duk
AU  - Park KD
FAU - Reese, Melissa
AU  - Reese M
FAU - Marti, Luciana
AU  - Marti L
FAU - Broadwater, Gloria
AU  - Broadwater G
FAU - Kurtzberg, Joanne
AU  - Kurtzberg J
LA  - eng
GR  - N01-HB-67138/HB/NHLBI NIH HHS/United States
GR  - N01-HB-67141/HB/NHLBI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - England
TA  - Stem Cells
JT  - Stem cells (Dayton, Ohio)
JID - 9304532
RN  - 0 (CD11 Antigens)
RN  - 0 (HLA-DR Antigens)
RN  - 0 (IL3RA protein, human)
RN  - 0 (Interleukin-3 Receptor alpha Subunit)
RN  - 0 (Receptors, Interleukin-3)
SB  - IM
MH  - Blood Cells/*cytology/immunology
MH  - Bone Marrow Cells/*cytology/immunology
MH  - CD11 Antigens/immunology
MH  - Cell Count/*methods
MH  - Cell Differentiation/immunology
MH  - Cell Lineage/immunology
MH  - Dendritic Cells/*cytology/immunology
MH  - Fetal Blood/*cytology/immunology
MH  - Flow Cytometry/methods
MH  - HLA-DR Antigens/immunology
MH  - Hematopoietic Stem Cells/cytology/immunology
MH  - Humans
MH  - Interleukin-3 Receptor alpha Subunit
MH  - Lymphocytes/cytology/immunology
MH  - Phenotype
MH  - Receptors, Interleukin-3/immunology
MH  - Reference Values
EDAT- 2003/05/14 05:00
MHDA- 2004/02/28 05:00
CRDT- 2003/05/14 05:00
PHST- 2003/05/14 05:00 [pubmed]
PHST- 2004/02/28 05:00 [medline]
PHST- 2003/05/14 05:00 [entrez]
AID - 10.1634/stemcells.21-3-296 [doi]
PST - ppublish
SO  - Stem Cells. 2003;21(3):296-303. doi: 10.1634/stemcells.21-3-296.