PMID- 12744739
OWN - NLM
STAT- MEDLINE
DCOM- 20040909
LR  - 20161018
IS  - 1009-2137 (Print)
IS  - 1009-2137 (Linking)
VI  - 11
IP  - 2
DP  - 2003 Apr
TI  - [Detection of 20q(-) chromosome abnormality in myelodysplastic syndrome by 
      interphase fluorescence in situ hybridization].
PG  - 165-8
AB  - In order to explore the value of interphase fluorescence in situ hybridization 
      (FISH) in the detection of partial deletion of the long arm of chromosome 20 
      (20q(-)) in patients with myelodysplastic syndrome (MDS), spectrum Green 
      fluorescein directly labeled yeast artificial chromosome (YAC) clone 912C3 which 
      spans the breakpoint cluster region in band 20q12 was used as probes to perform 
      interphase FISH on the marrow cells from 52 cases of MDS and 5 normal controls. 
      200 to 300 cells were scored for each case and cases which had cells with a green 
      hybridization signal>7.16% were defined as 20q(-) positive. The results of FISH 
      were compared with those of conventional cytogenetics (CC) assay. The results 
      showed that among 52 cases of MDS, 7 (13.5%) cases were positive by FISH, 
      however, of which, 4 cases were positive and the other 3 cases were negative by 
      CC assay. It is concluded that YAC912C3 and interphase FISH providing a powerful 
      technique in the detection of 20q(-) in MDS is an important complement to CC 
      assay.
FAU - Shen, Yong-Mei
AU  - Shen YM
AD  - The First Affiliated Hospital of Suzhou University, Jiangsu Institute of 
      Hematology, Suzhou 215006, China.
FAU - Xue, Yong-Quan
AU  - Xue YQ
FAU - Li, Jian-Yong
AU  - Li JY
FAU - Pan, Jin-Lan
AU  - Pan JL
FAU - Wu, Ya-Fang
AU  - Wu YF
FAU - Chen, Su-Ning
AU  - Chen SN
LA  - chi
PT  - English Abstract
PT  - Journal Article
PL  - China
TA  - Zhongguo Shi Yan Xue Ye Xue Za Zhi
JT  - Zhongguo shi yan xue ye xue za zhi
JID - 101084424
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Aged
MH  - *Chromosome Aberrations
MH  - *Chromosomes, Human, Pair 20
MH  - Female
MH  - Humans
MH  - *In Situ Hybridization, Fluorescence
MH  - *Interphase
MH  - Male
MH  - Middle Aged
MH  - Myelodysplastic Syndromes/*genetics
EDAT- 2003/05/15 05:00
MHDA- 2004/09/10 05:00
CRDT- 2003/05/15 05:00
PHST- 2003/05/15 05:00 [pubmed]
PHST- 2004/09/10 05:00 [medline]
PHST- 2003/05/15 05:00 [entrez]
AID - 1009-2137(2003)02-0165-04 [pii]
PST - ppublish
SO  - Zhongguo Shi Yan Xue Ye Xue Za Zhi. 2003 Apr;11(2):165-8.