PMID- 12745656
OWN - NLM
STAT- MEDLINE
DCOM- 20030717
LR  - 20091103
IS  - 1024-5332 (Print)
IS  - 1024-5332 (Linking)
VI  - 8
IP  - 2
DP  - 2003 Apr
TI  - New strategies for BMT, organ transplantation, and regeneration therapy.
PG  - 77-81
AB  - Bone marrow transplantation (BMT) is becoming a powerful strategy for the 
      treatment of hematologic disorders, congenital immunodeficiencies, metabolic 
      disorders and also autoimmune diseases. We have previously found using various 
      animal models for spontaneous autoimmune diseases, that allogeneic bone marrow 
      transplantation (allo BMT) can be used to prevent and treat various autoimmune 
      diseases. In addition, we have found that autoimmune diseases are stem cell 
      disorders. However, in MRL/lpr mice, which are radiosensitive (<8.5 Gy), we found 
      that conventional BMT had only a transient effect on autoimmune diseases, which 
      were found to recur. Therefore, we concentrated on discovering new strategies to 
      prevent and treat autoimmune diseases in the radiosensitive and 
      chimeric-resistant MRL/lpr mouse. Using MRL/lpr mice, we established a new method 
      for allo BMT. In this method, whole bone marrow cells (BMCs), containing a small 
      number of T cells and mesenchymal stem cells (MSCs), were directly injected into 
      the bone marrow cavity (intra-bone marrow [IBM]-BMT). MRL/lpr mice treated with 
      IBM-BMT survived more than 2 years without showing the symptoms of autoimmune 
      diseases. To apply this BMT method to humans, we have also established a new 
      method for BMC harvesting using cynomolgus monkeys. In this method, BMCs are 
      harvested from the long bones using a "Perfusion Method" (PM) and the whole BMCs 
      (including MSCs) are then injected directly into the IBM. We believe that this 
      new method will become a powerful strategy for the treatment of various 
      intractable diseases, including age-associated diseases such as osteoporosis.
FAU - Ikehara, Susumu
AU  - Ikehara S
AD  - First Department of Pathology, Transplantation Center, Regeneration Research 
      Center for intractable Diseases, Kansai Medical University, Osaka 570-8506, USA. 
      ikehara@takii.kmu.ac.jp
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Review
PL  - England
TA  - Hematology
JT  - Hematology (Amsterdam, Netherlands)
JID - 9708388
SB  - IM
MH  - Animals
MH  - Autoimmune Diseases/genetics/prevention & control/therapy
MH  - Bone Marrow
MH  - Bone Marrow Transplantation/*methods
MH  - Graft vs Host Disease/prevention & control
MH  - Humans
MH  - Immune Tolerance
MH  - Injections
MH  - Injections, Intravenous
MH  - Lupus Erythematosus, Systemic/genetics/prevention & control/therapy
MH  - Macaca fascicularis
MH  - Mice
MH  - Mice, Inbred MRL lpr
MH  - Models, Animal
MH  - Organ Transplantation/*methods
MH  - Osteoporosis/genetics/prevention & control
MH  - Portal Vein
MH  - Radiation Chimera
MH  - *Regeneration
MH  - Safety
MH  - Stem Cell Transplantation
MH  - Stromal Cells/transplantation
MH  - Tissue and Organ Harvesting/methods
MH  - Transplantation, Homologous
RF  - 38
EDAT- 2003/05/15 05:00
MHDA- 2003/07/18 05:00
CRDT- 2003/05/15 05:00
PHST- 2003/05/15 05:00 [pubmed]
PHST- 2003/07/18 05:00 [medline]
PHST- 2003/05/15 05:00 [entrez]
AID - FG1GHTD0UNHRM4FN [pii]
AID - 10.1080/1024533031000084240 [doi]
PST - ppublish
SO  - Hematology. 2003 Apr;8(2):77-81. doi: 10.1080/1024533031000084240.