PMID- 12748337
OWN - NLM
STAT- MEDLINE
DCOM- 20031014
LR  - 20190513
IS  - 0931-0509 (Print)
IS  - 0931-0509 (Linking)
VI  - 18
IP  - 6
DP  - 2003 Jun
TI  - Increased production of chemotactic cytokines and elevated proliferation and 
      expression of intercellular adhesion molecule-1 in rat mesangial cells treated 
      with erythrogenic toxin type B and its precursor isolated from nephritogenic 
      streptococci.
PG  - 1072-8
AB  - BACKGROUND: Previous reports have demonstrated the presence of streptococcal 
      erythrogenic toxin type B (ETB) as well as proliferation and expression of 
      adhesion molecules along with leukocyte infiltrations in biopsies from patients 
      with acute post-streptococcal glomerulonephritis (APSGN). The purpose of the 
      present study was to correlate infiltrative and proliferative events with 
      interactions between ETB or its precursor (ETBP) and intrinsic mesangial cells. 
      METHODS: Rat mesangial cells were cultured with ETB or ETBP (50 micro g/ml) while 
      measuring production of monocyte chemoattractant protein-1 (MCP-1) and macrophage 
      inflammatory protein-2 (MIP-2) and while examining proliferation and expression 
      of intercellular adhesion molecule-1 (ICAM-1). After 24, 48 and 96 h of 
      incubation, MCP-1 and MIP-2 in culture supernatants were assessed by 
      enzyme-linked immunosorbent assay (ELISA). Cells were assessed for proliferation 
      by incorporation of radioactive thymidine and expression of ICAM-1 was measured 
      by indirect immunofluorescence and by cellular ELISA. RESULTS: Compared with 
      controls, treatment with either ETBP or ETB significantly increased MCP-1 and 
      MIP-2 levels in mesangial cell cultures. Mesangial cells also showed elevated 
      proliferation at 96 h of culture when treated with streptococcal proteins. 
      Although production of MCP-1 and MIP-2 was not correlated with proliferation, 
      treatment with ETBP resulted in a significant correlation between MCP-1 
      production and proliferation. Immunofluorescence studies revealed an increased 
      expression of ICAM-1 in ETBP/ETB-treated mesangial cells. In addition, cellular 
      ELISA studies showed increased absorbance in cultures treated with ETBP/ETB. 
      Finally, low serum concentrations in the culture medium potentiated the 
      stimulatory effect of ETB on MCP-1 production. CONCLUSIONS: Our findings, by 
      demonstrating a role for cationic streptococcal ETB or ETBP in the induction of 
      chemotactic molecules as well as the proliferation and expression of adhesion 
      molecules, delineate an additional possible pathway for the pathogenesis of 
      APSGN.
FAU - Rincon, Jaimar
AU  - Rincon J
AD  - Instituto de Investigaciones Clinicas Dr. Americo Negrette, Facultad de Medicina, 
      Universidad del Zulia, Maracaibo, Venezuela.
FAU - Viera, Ninoska T
AU  - Viera NT
FAU - Romero, Maritza J
AU  - Romero MJ
FAU - Mosquera, Jesus A
AU  - Mosquera JA
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Nephrol Dial Transplant
JT  - Nephrology, dialysis, transplantation : official publication of the European 
      Dialysis and Transplant Association - European Renal Association
JID - 8706402
RN  - 0 (Bacterial Proteins)
RN  - 0 (Chemokine CCL2)
RN  - 0 (Chemokine CXCL2)
RN  - 0 (Exotoxins)
RN  - 0 (Membrane Proteins)
RN  - 0 (Monokines)
RN  - 0 (SpeA protein, Streptococcus pyogenes)
RN  - 0 (erythrogenic toxin)
RN  - 126547-89-5 (Intercellular Adhesion Molecule-1)
SB  - IM
MH  - Animals
MH  - *Bacterial Proteins
MH  - Chemokine CCL2/*biosynthesis
MH  - Chemokine CXCL2
MH  - Disease Models, Animal
MH  - Exotoxins/*pharmacology
MH  - Glomerulonephritis/immunology/metabolism/microbiology
MH  - Intercellular Adhesion Molecule-1/*biosynthesis
MH  - Kidney/cytology/*drug effects/immunology
MH  - Male
MH  - *Membrane Proteins
MH  - Monokines/*biosynthesis
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - *Streptococcus
EDAT- 2003/05/16 05:00
MHDA- 2003/10/15 05:00
CRDT- 2003/05/16 05:00
PHST- 2003/05/16 05:00 [pubmed]
PHST- 2003/10/15 05:00 [medline]
PHST- 2003/05/16 05:00 [entrez]
AID - 10.1093/ndt/gfg109 [doi]
PST - ppublish
SO  - Nephrol Dial Transplant. 2003 Jun;18(6):1072-8. doi: 10.1093/ndt/gfg109.