PMID- 12749944
OWN - NLM
STAT- MEDLINE
DCOM- 20030903
LR  - 20190823
IS  - 0093-691X (Print)
IS  - 0093-691X (Linking)
VI  - 60
IP  - 2
DP  - 2003 Jul
TI  - Immunization against 5alpha-androstane-3alpha,17beta-diol affects ovarian 
      folliculogenesis in Merino ewes.
PG  - 307-17
AB  - The 5alpha-reduced androgens have been implicated as antagonists of follicular 
      development. In this experiment, we examined the effect of active immunization 
      against 5alpha-reduced androgen on follicular development in ewes. During the 
      breeding season, cyclic Merino ewes were either actively immunized three times 
      against 5alpha-androstane-3alpha,17beta-diol (3alpha-diol) or served as controls. 
      Six to nine weeks after the last immunization, they were treated with PGF(2alpha) 
      analog (PG, 125mg cloprostenol i.m.) and luteolysis was induced. Fourteen days 
      after the PG treatment, the ewes were either killed (mid-luteal phase) or treated 
      a second time with PG and killed 24h later (early follicular phase). At 
      slaughter, blood samples were collected and ovaries recovered. All CL and 
      follicles larger than 2mm were dissected and their size and appearance were 
      recorded. Follicular fluid was collected and concentrations of estradiol-17beta 
      (E(2)), progesterone (P), androstenedione (A(4)), testosterone (T), 
      dihydrotestosterone (DHT), 5alpha-androstane-3alpha-ol,17beta-one (androsterone: 
      3alpha-ol) and 3alpha-diol were determined by RIA. Immunization induced 
      antibodies primarily to DHT and its 5alpha-reduced substrates 3alpha-diol and 
      3alpha-ol but not to E(2), P, A(4) or T. Immunization increased ovulation rate, 
      size of ovulatory follicles and weight of CL. Immunization appeared to increase 
      ovulation rate by decreasing the incidence of atresia in large preovulatory 
      follicles. Regardless of their physiological status follicles contained only low 
      levels of DHT; 3alpha-ol and 3alpha-diol were not detected in most follicles. 
      Immunization did not appear to affect levels of DHT or other steroids in the 
      follicular fluid. In conclusion, the induction of antibodies to 5alpha-reduced 
      androgens increases ovulation rate by enhancing follicular viability during the 
      preovulatory period in ewes. However, this effect is not brought about by the 
      direct immune-neutralization of DHT or its 5alpha-reduced substrates 3alpha-ol 
      and 3alpha-diol at the ovarian level.
FAU - Tetsuka, Masa
AU  - Tetsuka M
AD  - Department of Wool and Animal Science, The University of New South Wales, P.O. 
      Box 1, Kensington, Australia. mtetsuka@obihiro.ac.jp
FAU - Nancarrow, Colin D
AU  - Nancarrow CD
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Theriogenology
JT  - Theriogenology
JID - 0421510
RN  - 0 (Antibodies)
RN  - 08J2K08A3Y (Dihydrotestosterone)
RN  - 25126-76-5 (Androstane-3,17-diol)
RN  - 3XMK78S47O (Testosterone)
RN  - 409J2J96VR (Androstenedione)
RN  - 4208238832 (Cloprostenol)
RN  - 4G7DS2Q64Y (Progesterone)
RN  - 4TI98Z838E (Estradiol)
RN  - C24W7J5D5R (Androsterone)
SB  - IM
MH  - Androstane-3,17-diol/analysis/*immunology
MH  - Androstenedione/analysis
MH  - Androsterone/analysis/immunology
MH  - Animals
MH  - Antibodies/blood
MH  - Breeding
MH  - Cloprostenol/administration & dosage
MH  - Corpus Luteum/physiology
MH  - Dihydrotestosterone/analysis/immunology
MH  - Estradiol/analysis
MH  - Female
MH  - Follicular Fluid/chemistry
MH  - Immunization/*veterinary
MH  - Ovarian Follicle/*physiology
MH  - Ovulation
MH  - Progesterone/analysis
MH  - Seasons
MH  - Sheep/*physiology
MH  - Testosterone/analysis
EDAT- 2003/05/17 05:00
MHDA- 2003/09/04 05:00
CRDT- 2003/05/17 05:00
PHST- 2003/05/17 05:00 [pubmed]
PHST- 2003/09/04 05:00 [medline]
PHST- 2003/05/17 05:00 [entrez]
AID - S0093691X02013705 [pii]
AID - 10.1016/s0093-691x(02)01370-5 [doi]
PST - ppublish
SO  - Theriogenology. 2003 Jul;60(2):307-17. doi: 10.1016/s0093-691x(02)01370-5.