PMID- 12750341
OWN - NLM
STAT- MEDLINE
DCOM- 20040130
LR  - 20210915
IS  - 0016-6731 (Print)
IS  - 0016-6731 (Linking)
VI  - 164
IP  - 1
DP  - 2003 May
TI  - Cloning and characterization of maize B chromosome sequences derived from 
      microdissection.
PG  - 299-310
AB  - Isolation of sequences from the maize B chromosome is always hampered by its high 
      homology with the normal complements. In this study, this handicap was overcome 
      by cloning the sequences from the pachytene B chromosomes dissected out of a 
      slide by a micromanipulator followed by degenerate oligonucleotide-primed PCR. 
      The isolated sequences were found to hybridize with genomic DNA in a 
      B-dosage-dependent manner and with the pachytene B chromosome by fluorescence in 
      situ hybridization (FISH), corroborating their B origin. A total of 19 B 
      sequences were isolated, all of which are repetitive and, with one exception, are 
      homologous to the A chromosome(s). Three sequences have strong homology to maize 
      sequences that include two knob repeats and one zein gene (noncoding region), and 
      10 others are homologous to the noncoding region of Adh1, Bz1, Gag, Zein, and B 
      centromere to a lesser degree. Six sequences have no homology to any gene. In 
      addition to FISH, the B-specific sequence and a partially B-specific one were 
      also mapped, by seven newly characterized TB-10L translocations, to a similar 
      location on the central portion of the distal heterochromatic region, spreading 
      over a region of about one-third of the B chromosome.
FAU - Cheng, Ya-Ming
AU  - Cheng YM
AD  - Institute of Molecular Biology, National Chung Hsing University, Taichung 402, 
      Taiwan, Republic of China.
FAU - Lin, Bor-Yaw
AU  - Lin BY
LA  - eng
SI  - GENBANK/BH814952
SI  - GENBANK/BH814953
SI  - GENBANK/BH814954
SI  - GENBANK/BH814955
SI  - GENBANK/BH814957
SI  - GENBANK/BH814958
SI  - GENBANK/BH814959
SI  - GENBANK/BH814960
SI  - GENBANK/BH814961
SI  - GENBANK/BH814962
SI  - GENBANK/BH814963
SI  - GENBANK/BH814964
SI  - GENBANK/BH814965
SI  - GENBANK/BH814966
SI  - GENBANK/BH814967
SI  - GENBANK/BH814968
SI  - GENBANK/BH814969
SI  - GENBANK/BH814970
SI  - GENBANK/BH814971
SI  - GENBANK/BH814972
SI  - GENBANK/BH814973
SI  - GENBANK/BH814974
SI  - GENBANK/BH814975
SI  - GENBANK/BH814976
SI  - GENBANK/BH814977
PT  - Journal Article
PL  - United States
TA  - Genetics
JT  - Genetics
JID - 0374636
SB  - IM
MH  - Blotting, Southern
MH  - *Chromosomes
MH  - In Situ Hybridization, Fluorescence
MH  - *Microdissection
MH  - Molecular Sequence Data
MH  - *Sequence Analysis, DNA
MH  - Translocation, Genetic
MH  - Zea mays/*genetics
PMC - PMC1462555
EDAT- 2003/05/17 05:00
MHDA- 2004/01/31 05:00
PMCR- 2003/08/01
CRDT- 2003/05/17 05:00
PHST- 2003/05/17 05:00 [pubmed]
PHST- 2004/01/31 05:00 [medline]
PHST- 2003/05/17 05:00 [entrez]
PHST- 2003/08/01 00:00 [pmc-release]
AID - 10.1093/genetics/164.1.299 [doi]
PST - ppublish
SO  - Genetics. 2003 May;164(1):299-310. doi: 10.1093/genetics/164.1.299.