PMID- 12750998
OWN - NLM
STAT- MEDLINE
DCOM- 20030806
LR  - 20181113
IS  - 0171-5216 (Print)
IS  - 0171-5216 (Linking)
VI  - 129
IP  - 5
DP  - 2003 May
TI  - The abnormalities of chromosome 8 in two hepatocellular carcinoma cell clones 
      with the same genetic background and different metastatic potential.
PG  - 303-8
AB  - PURPOSE: Two hepatocellular carcinoma (HCC) cell clones named MHCC97-H and 
      MHCC97-L with different metastatic potential have recently been established from 
      the same parent cell line MHCC97 in our institute. The cytogenetic alterations of 
      these two clones were investigated in this study to explore the possible clues to 
      the mechanism involved in HCC metastasis. METHODS: Their chromosomal aberrations 
      were analyzed with comparative genomic hybridization (CGH), chromosome-specific 
      painting, and two-color fluorescence in situ hybridization (FISH). RESULTS: The 
      aberrations were found in a total of 17 chromosomes, and six kinds of the 
      aberrations including gains of 1q, 7q, 8q, 20, and the losses of 8p23, 21q were 
      found both in the two cell clones and their parent cell line MHCC97. Using 
      modified CGH, with the DNA of MHCC97-L as control to test the MHCC97-H clone, the 
      loss of 8p23 and the gain of 1q31-32, 8q21.3-22, 13q22, 17q22 were highlighted, 
      and the most significant finding was on chromosome 8. Dual color FISH combining a 
      pericentromeric probe and a BAC probe mapping at 8q23.1 was then performed to 
      verify this result, and the signal ratios of the BAC to centromere were 1.43 in 
      MHCC97-H and 1.45 in MHCC97-L, confirming the over-representations at 8q in both 
      cells. Another interesting finding in the dual-color metaphase FISH was the 
      intrachromosomal translocation of 8q to 8p (looked like an isochromosome 8) and 
      non-reciprocal translocation of part of 8q to 4q, which was further clarified and 
      proved by the FISH with whole chromosome 8 painting probe. CONCLUSIONS: The high 
      copies amplification on 8q, the formation of isochromosome 8, non-reciprocal 
      translocation of partial 8q to 4q, and loss of 8p occurred at the same time and 
      are the characteristic chromosomal aberrations of the two cell clones. The 
      chromosome 8p, especially 8p23, might harbor some novel genes related to the HCC 
      metastasis.
FAU - Yang, Jiong
AU  - Yang J
AD  - Liver Cancer Institute & Zhongshan Hospital of Fudan University, 136 Yi Xue Yuan 
      Road, 200032, Shanghai, China.
FAU - Qin, Lun-Xiu
AU  - Qin LX
FAU - Ye, Sheng-Long
AU  - Ye SL
FAU - Liu, Yin-Kun
AU  - Liu YK
FAU - Li, Yan
AU  - Li Y
FAU - Gao, Dong-Mei
AU  - Gao DM
FAU - Chen, Jie
AU  - Chen J
FAU - Tang, Zhao-You
AU  - Tang ZY
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20030515
PL  - Germany
TA  - J Cancer Res Clin Oncol
JT  - Journal of cancer research and clinical oncology
JID - 7902060
SB  - IM
MH  - Carcinoma, Hepatocellular/*genetics/secondary
MH  - *Chromosome Aberrations
MH  - Chromosome Painting/methods
MH  - Chromosomes, Human, Pair 8/*genetics
MH  - Cloning, Molecular
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/methods
MH  - Liver Neoplasms/*genetics/pathology
MH  - Nucleic Acid Hybridization/methods
MH  - Tumor Cells, Cultured
EDAT- 2003/05/17 05:00
MHDA- 2003/08/07 05:00
CRDT- 2003/05/17 05:00
PHST- 2002/12/21 00:00 [received]
PHST- 2003/01/28 00:00 [accepted]
PHST- 2003/05/17 05:00 [pubmed]
PHST- 2003/08/07 05:00 [medline]
PHST- 2003/05/17 05:00 [entrez]
AID - 10.1007/s00432-003-0436-8 [doi]
PST - ppublish
SO  - J Cancer Res Clin Oncol. 2003 May;129(5):303-8. doi: 10.1007/s00432-003-0436-8. 
      Epub 2003 May 15.