PMID- 12752058
OWN - NLM
STAT- MEDLINE
DCOM- 20030729
LR  - 20190922
IS  - 1395-3907 (Print)
IS  - 1395-3907 (Linking)
VI  - 81
IP  - 2
DP  - 2003 Apr
TI  - Effects of topical vitamin E on corneal superoxide dismutase, glutathione 
      peroxidase activities and polymorphonuclear leucocyte infiltration after 
      photorefractive keratectomy.
PG  - 177-80
AB  - PURPOSE: Photorefractive keratectomy (PRK) induces free radical formation and 
      polymorphonuclear (PMN) cell infiltration in the cornea. Vitamin E is a free 
      radical scavenger and protects the cells from reactive oxygen species. We 
      investigated the effects of topical vitamin E on corneal PMN cell infiltration 
      and corneal antioxidant enzyme activities after PRK. METHODS: We studied four 
      groups, each consisting of seven eyes. Group 1 were control eyes. In group 2 the 
      corneal epithelium was removed by a blunt spatula (epithelial scrape). In group 
      3, corneal photoablation (59 micro m, 5 dioptres) was performed after epithelial 
      removal (traditional PRK). In group 4 we tested the effects of topical Vitamin E 
      after traditional PRK. Corneal tissues were removed and studied with enzymatic 
      analysis (measurement of corneal superoxide dismutase and glutathione peroxidase 
      activities) and histologically. RESULTS: Stromal PMN leucocyte counts were 
      significantly higher after mechanical epithelial removal and traditional PRK (p < 
      0.05). Corneal superoxide dismutase and glutathione peroxidase activities 
      decreased significantly after mechanical epithelial removal and traditional PRK 
      (p < 0.05). In group 4, treated with vitamin E, corneal superoxide dismutase 
      activity did not differ significantly from that in the medically non-treated 
      groups, nor did corneal PMN cell infiltration after traditional PRK. The 
      reduction of corneal glutathione peroxidase activity after PRK was reduced 
      significantly after topical vitamin E treatment. CONCLUSIONS: Topical vitamin E 
      treatment may be useful for reducing the harmful effects of reactive oxygen 
      radical after epithelial scraping and PRK in that it increases corneal 
      glutathione peroxidase activity.
FAU - Bilgihan, Ayse
AU  - Bilgihan A
AD  - Department of Biochemistry, Gazi University Medical School, Ankara, Turkey. 
      ayseb@med.gazi.edu.tr
FAU - Bilgihan, Kamil
AU  - Bilgihan K
FAU - Yis, Ozgur
AU  - Yis O
FAU - Sezer, Cem
AU  - Sezer C
FAU - Akyol, Gulen
AU  - Akyol G
FAU - Hasanreisoglu, Berati
AU  - Hasanreisoglu B
LA  - eng
PT  - Journal Article
PL  - Denmark
TA  - Acta Ophthalmol Scand
JT  - Acta ophthalmologica Scandinavica
JID - 9507578
RN  - 0 (Antioxidants)
RN  - 1406-18-4 (Vitamin E)
RN  - EC 1.11.1.9 (Glutathione Peroxidase)
RN  - EC 1.15.1.1 (Superoxide Dismutase)
SB  - IM
MH  - Administration, Topical
MH  - Animals
MH  - Antioxidants/*administration & dosage
MH  - Cornea/drug effects/*enzymology/pathology
MH  - Glutathione Peroxidase/*metabolism
MH  - Lasers, Excimer
MH  - Leukocyte Count
MH  - Neutrophil Infiltration/*drug effects
MH  - Neutrophils/pathology
MH  - *Photorefractive Keratectomy
MH  - *Postoperative Care
MH  - Rabbits
MH  - Superoxide Dismutase/*metabolism
MH  - Vitamin E/*administration & dosage
EDAT- 2003/05/20 05:00
MHDA- 2003/07/30 05:00
CRDT- 2003/05/20 05:00
PHST- 2003/05/20 05:00 [pubmed]
PHST- 2003/07/30 05:00 [medline]
PHST- 2003/05/20 05:00 [entrez]
AID - 042 [pii]
AID - 10.1034/j.1600-0420.2003.00042.x [doi]
PST - ppublish
SO  - Acta Ophthalmol Scand. 2003 Apr;81(2):177-80. doi: 
      10.1034/j.1600-0420.2003.00042.x.