PMID- 12752327
OWN - NLM
STAT- MEDLINE
DCOM- 20031110
LR  - 20190823
IS  - 0105-4538 (Print)
IS  - 0105-4538 (Linking)
VI  - 58
IP  - 5
DP  - 2003 May
TI  - Polygalacturonase (pectinase), a new oilseed rape allergen.
PG  - 407-11
AB  - BACKGROUND: Type I hypersensitivity to rapeseed pollen allergens was described as 
      the result of a cross-sensitization with various pollens that could constitute an 
      aggravating factor in birch or grass pollen allergies. Recently, a few rapeseed 
      pollen allergens were described. The aim of the present work was to identify new 
      rapeseed pollen allergens by using two-dimensional gel analysis, microsequencing, 
      and mass spectrometry. METHODS: Water extractable proteins from oilseed rape 
      pollen or stamen were separated by two-dimensional gel electrophoresis. The 
      proteins were then electroblotted onto a nitrocellulose (NC) sheet. The NC sheets 
      were successively incubated with (1) individual human sera pre-selected for their 
      immunoglobulin E (IgE) reactivity to rapeseed pollen proteins, (2) alkaline 
      phosphatase (AP)-conjugated goat anti-human IgE and (3) AP substrate. The 
      allergens localized by this method were then identified by microsequencing and 
      MALDI-TOF mass spectrometry analysis. RESULTS: Of the 18 sera studied, five 
      recognized a wide multispot zone with a molecular mass around 43 kD and pIs 
      between 6.5 and 8.5. The results obtained with two representative sera are shown. 
      From this zone, two isoforms of the polygalacturonase enzyme were identified by 
      microsequencing. Confirmation was obtained through MALDI-TOF mass spectrometry 
      analysis. CONCLUSION: The present results allow the identification of a new 
      rapeseed allergen that can be the main allergen for some patients.
FAU - Chardin, H
AU  - Chardin H
AD  - Institut Pasteur, Paris, France; Universite Rene Descartes, Paris, France.
FAU - Mayer, C
AU  - Mayer C
FAU - Senechal, H
AU  - Senechal H
FAU - Poncet, P
AU  - Poncet P
FAU - Clement, G
AU  - Clement G
FAU - Wal, J M
AU  - Wal JM
FAU - Desvaux, F X
AU  - Desvaux FX
FAU - Peltre, G
AU  - Peltre G
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Denmark
TA  - Allergy
JT  - Allergy
JID - 7804028
RN  - 0 (Allergens)
RN  - 0 (Fatty Acids, Monounsaturated)
RN  - 0 (Isoenzymes)
RN  - 0 (Plant Oils)
RN  - 0 (Plant Proteins)
RN  - 0 (Rapeseed Oil)
RN  - 37341-29-0 (Immunoglobulin E)
RN  - EC 3.2.1.15 (Polygalacturonase)
SB  - IM
MH  - Allergens/analysis/*immunology
MH  - Blotting, Western
MH  - Brassica rapa/*immunology
MH  - Electrophoresis, Gel, Two-Dimensional
MH  - Fatty Acids, Monounsaturated
MH  - Humans
MH  - Hypersensitivity, Immediate/immunology
MH  - Immunoglobulin E/blood/immunology
MH  - Isoenzymes/analysis
MH  - Mass Spectrometry
MH  - *Plant Oils
MH  - Plant Proteins/immunology
MH  - Pollen/*immunology
MH  - Polygalacturonase/*immunology
MH  - Rapeseed Oil
EDAT- 2003/05/20 05:00
MHDA- 2003/11/11 05:00
CRDT- 2003/05/20 05:00
PHST- 2003/05/20 05:00 [pubmed]
PHST- 2003/11/11 05:00 [medline]
PHST- 2003/05/20 05:00 [entrez]
AID - 094 [pii]
AID - 10.1034/j.1398-9995.2003.00094.x [doi]
PST - ppublish
SO  - Allergy. 2003 May;58(5):407-11. doi: 10.1034/j.1398-9995.2003.00094.x.