PMID- 12753554
OWN - NLM
STAT- MEDLINE
DCOM- 20030710
LR  - 20191107
IS  - 0936-6768 (Print)
IS  - 0936-6768 (Linking)
VI  - 38
IP  - 3
DP  - 2003 Jun
TI  - Manipulated mouse embryos as bioassay system for water quality control.
PG  - 204-8
AB  - Mouse pronuclear stage embryos with intact slit zona pellucida (manipulated) were 
      cultured in vitro until the hatched blastocyst stage in simplex optimized medium 
      with higher K+ concentration (KSOM) prepared with three different water types: 
      tap, deionized reverse osmosis (D-O) water and Milli-Q system (M-Q) water. The 
      culture media were supplemented with or without protein and 
      ethylenediaminetetraacetic acid (EDTA, disodium salt). The rates of hatched 
      blastocysts were significantly affected (p < 0.01) by micromanipulation, protein 
      supplement and water source. The water source has no influence (p > 0.05) on 
      development in EDTA-supplemented protein-free culture media, whereas in EDTA- and 
      protein-free culture media, the water quality significantly (p < 0.001) affected 
      the rates of development, with higher rates in media prepared with M-Q water. The 
      micromanipulated embryos showed higher sensitivity to the water quality (p < 
      0.01). It worth mentioning that the rates of hatched blastocysts in protein-free 
      culture media were very low (0-7.5%). Furthermore, the three different water 
      types were analysed by measuring the electrical conductivity, inorganic ions, 
      total organic carbon and endotoxins to evaluate the purity. M-Q water showed the 
      lowest levels of inorganic ion, total organic carbon and endotoxin 
      concentrations. We concluded that manipulated mouse embryos are good system to 
      evaluate the quality of water used in biological system.
FAU - Elsheikh, A S
AU  - Elsheikh AS
AD  - Laboratory of Theriogenology, Department of Veterinary Clinical Sciences, 
      Graduate School of Veterinary Medicine, Hokkaido University, Sapporo, Japan.
FAU - Takahashi, Y
AU  - Takahashi Y
FAU - Nagano, M
AU  - Nagano M
FAU - Kanagawa, H
AU  - Kanagawa H
LA  - eng
PT  - Journal Article
PL  - Germany
TA  - Reprod Domest Anim
JT  - Reproduction in domestic animals = Zuchthygiene
JID - 9015668
RN  - 0 (Culture Media)
RN  - 0 (Ions)
RN  - 0 (Proteins)
RN  - 9G34HU7RV0 (Edetic Acid)
SB  - IM
MH  - Animals
MH  - Blastocyst/*physiology
MH  - Culture Media
MH  - Edetic Acid
MH  - Electric Conductivity
MH  - Female
MH  - Fertilization in Vitro/*standards
MH  - Ions
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Micromanipulation
MH  - Pregnancy
MH  - Proteins
MH  - Quality Control
MH  - Water Supply/*standards
EDAT- 2003/05/20 05:00
MHDA- 2003/07/11 05:00
CRDT- 2003/05/20 05:00
PHST- 2003/05/20 05:00 [pubmed]
PHST- 2003/07/11 05:00 [medline]
PHST- 2003/05/20 05:00 [entrez]
AID - 427 [pii]
AID - 10.1046/j.1439-0531.2003.00427.x [doi]
PST - ppublish
SO  - Reprod Domest Anim. 2003 Jun;38(3):204-8. doi: 10.1046/j.1439-0531.2003.00427.x.