PMID- 12753658
OWN - NLM
STAT- MEDLINE
DCOM- 20040129
LR  - 20190826
IS  - 0001-2815 (Print)
IS  - 0001-2815 (Linking)
VI  - 61
IP  - 5
DP  - 2003 May
TI  - TNF-alpha polymorphisms and type 2 diabetes mellitus in Taiwanese patients.
PG  - 393-7
AB  - Type 2 diabetic mellitus (type 2 DM) comprises more than 95% of all Taiwanese 
      patients with DM. Tumor necrosis factor-alpha (TNF-alpha) expression is linked 
      with insulin resistance, and is under strong genetic control. The correlation 
      between TNF promoter genotypes and type 2 DM is still controversial, because 
      discrepancies among different studies exist. Ethnic differences play certain 
      roles in these conflicting results, because the distribution of TNF promoter 
      polymorphisms is different among study subjects with different racial origins. 
      Therefore, we examined the relationship between the incidence of type 2 diabetes 
      in Taiwanese and two polymorphisms of the TNF-alpha promoter region (positions 
      -238 and -308) as well as the correlation between these polymorphisms and the 
      patients' biochemical manifestations. Genomic DNA was extracted from peripheral 
      blood cells of 261 Taiwanese patients with type 2 DM and 189 non-diabetic control 
      study subjects, and their TNF promoter G-238A and G-308A polymorphisms were 
      analyzed by PCR-RFLP analysis. No significant association between TNF-alpha 
      G-238A and G-308A polymorphisms with type 2 diabetic incidence was observed. 
      However, associations between TNF-alpha G-238A and low-density 
      lipoprotein-cholesterol and between G-308A promoter polymorphism and high-fasting 
      plasma glucose levels, using multiple linear regression analysis with adjustment 
      for the subjects' age, sex, body mass index and diabetic status, were found. Our 
      results suggested that though TNF-alpha G-238A and G-308A polymorphisms were not 
      involved in the pathogenesis of type 2 DM, type 2 diabetic patients carrying 
      TNFA-A or TNF-308*2 genotype might be more susceptible to diabetic complications 
      such as atherosclerosis.
FAU - Shiau, M-Y
AU  - Shiau MY
AD  - Hung Kuang University, Institute of Medicine, and Institute of Immunology, Chung 
      Shan Medical University, Taichung, Taiwan, Republic of China.
FAU - Wu, C-Y
AU  - Wu CY
FAU - Huang, C-N
AU  - Huang CN
FAU - Hu, S-W
AU  - Hu SW
FAU - Lin, S-J
AU  - Lin SJ
FAU - Chang, Y-H
AU  - Chang YH
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Tissue Antigens
JT  - Tissue antigens
JID - 0331072
RN  - 0 (Blood Glucose)
RN  - 0 (Cholesterol, LDL)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 9007-49-2 (DNA)
SB  - IM
MH  - Blood Glucose/metabolism
MH  - Body Mass Index
MH  - Case-Control Studies
MH  - Cholesterol, LDL/metabolism
MH  - DNA/genetics
MH  - Diabetes Mellitus, Type 2/epidemiology/*genetics
MH  - Disease Progression
MH  - Female
MH  - Genotype
MH  - Glucose Intolerance/*genetics
MH  - Humans
MH  - Insulin Resistance/*genetics
MH  - Male
MH  - Middle Aged
MH  - Polymerase Chain Reaction
MH  - *Polymorphism, Genetic
MH  - Polymorphism, Single-Stranded Conformational
MH  - *Promoter Regions, Genetic
MH  - Taiwan/epidemiology
MH  - Tumor Necrosis Factor-alpha/*genetics
EDAT- 2003/05/20 05:00
MHDA- 2004/01/30 05:00
CRDT- 2003/05/20 05:00
PHST- 2003/05/20 05:00 [pubmed]
PHST- 2004/01/30 05:00 [medline]
PHST- 2003/05/20 05:00 [entrez]
AID - 059 [pii]
AID - 10.1034/j.1399-0039.2003.00059.x [doi]
PST - ppublish
SO  - Tissue Antigens. 2003 May;61(5):393-7. doi: 10.1034/j.1399-0039.2003.00059.x.