PMID- 12754176
OWN - NLM
STAT- MEDLINE
DCOM- 20040408
LR  - 20130926
IS  - 8750-7587 (Print)
IS  - 0161-7567 (Linking)
VI  - 95
IP  - 3
DP  - 2003 Sep
TI  - C-reactive protein correlates with macrophage accumulation in coronary arteries 
      of hypercholesterolemic pigs.
PG  - 1301-4
AB  - A growing body of evidence supports the hypothesis that C-reactive protein (CRP) 
      is a marker of inflammation in coronary artery disease. The purpose of the 
      present study was to test the hypothesis that CRP correlates with macrophage 
      accumulation during the initial stages of coronary vascular disease. Adult male 
      pigs were fed a normal chow (NF) or a high-fat high-cholesterol (HF) diet for 20 
      wk. After 20 wk, blood was collected for analyses of interleukin-6 (IL-6), CRP, 
      and lipids. After blood collection, the pigs were euthanized and the right 
      coronary arteries (RCA) were harvested and fixed in neutral buffered formalin. 
      Paraffin-embedded sections of RCA were stained immunohistochemically for CRP, 
      scavenger receptor A (SRA), and monocyte chemoattractant protein-1 (MCP-1). All 
      cholesterol fractions were elevated in the HF vs. the NF group (P < 0.05). There 
      was little or no positive staining for CRP, SRA, or MCP-1 in the RCA of NF pigs, 
      but there was extensive staining in lipidladen macrophage foam cells in the HF 
      pigs. Double staining revealed colocalization of CRP with SRA and CRP with MCP-1 
      in foam cells. Serum IL-6 was below the assay detection limit in all pigs. Serum 
      CRP correlated directly with plasma total cholesterol (R = 0.727, P = 0.041) and 
      accumulation of SRA-positive macrophages (R = 0.938, P < 0.001) in RCA of HF 
      pigs. We conclude that serum CRP correlates with macrophage accumulation and 
      coronary artery disease in hypercholesterolemic pigs.
FAU - Turk, James R
AU  - Turk JR
AD  - Department of Biomedical Sciences, University of Missouri, Columbia, MO 65211, 
      USA. turkj@missouri.edu
FAU - Carroll, Jeffery A
AU  - Carroll JA
FAU - Laughlin, M Harold
AU  - Laughlin MH
FAU - Thomas, Tom R
AU  - Thomas TR
FAU - Casati, Jennifer
AU  - Casati J
FAU - Bowles, Douglas K
AU  - Bowles DK
FAU - Sturek, Michael
AU  - Sturek M
LA  - eng
GR  - HL-52490/HL/NHLBI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
DEP - 20030516
PL  - United States
TA  - J Appl Physiol (1985)
JT  - Journal of applied physiology (Bethesda, Md. : 1985)
JID - 8502536
RN  - 0 (Chemokine CCL2)
RN  - 0 (Cholesterol, Dietary)
RN  - 0 (Membrane Proteins)
RN  - 0 (Receptors, Immunologic)
RN  - 0 (Receptors, Lipoprotein)
RN  - 0 (Receptors, Scavenger)
RN  - 0 (Scarb1 protein, mouse)
RN  - 0 (Scavenger Receptors, Class B)
RN  - 9007-41-4 (C-Reactive Protein)
SB  - IM
MH  - Animals
MH  - Body Weight/physiology
MH  - C-Reactive Protein/*metabolism
MH  - Chemokine CCL2/metabolism
MH  - Cholesterol, Dietary/pharmacology
MH  - Coronary Vessels/*pathology
MH  - Hypercholesterolemia/*metabolism/*pathology
MH  - Immunohistochemistry
MH  - Macrophages/metabolism/*physiology
MH  - Male
MH  - *Membrane Proteins
MH  - Receptors, Immunologic/metabolism
MH  - *Receptors, Lipoprotein
MH  - Receptors, Scavenger
MH  - Scavenger Receptors, Class B
MH  - Swine
MH  - Swine, Miniature
EDAT- 2003/05/20 05:00
MHDA- 2004/04/09 05:00
CRDT- 2003/05/20 05:00
PHST- 2003/05/20 05:00 [pubmed]
PHST- 2004/04/09 05:00 [medline]
PHST- 2003/05/20 05:00 [entrez]
AID - 00342.2003 [pii]
AID - 10.1152/japplphysiol.00342.2003 [doi]
PST - ppublish
SO  - J Appl Physiol (1985). 2003 Sep;95(3):1301-4. doi: 
      10.1152/japplphysiol.00342.2003. Epub 2003 May 16.