PMID- 12755280
OWN - NLM
STAT- MEDLINE
DCOM- 20030905
LR  - 20051116
IS  - 0125-1562 (Print)
IS  - 0125-1562 (Linking)
VI  - 33 Suppl 2
DP  - 2002
TI  - Histocompatibility testing.
PG  - 112-4
AB  - The human leukocyte antigen (HLA) region on chromosome 6p21.3 is the most 
      polymorphic in the human genome. It encodes hundreds of genes, of which the class 
      I and class II HLA alleles play a central role in the generation of an immune 
      response, but at the same time represent a barrier to marrow and organ 
      transplantation. There is overwhelming evidence that optimal HLA matching will 
      allow for safer marrow transplants, however with more than 1,200 allelic 
      variants, the need to accurately identify the precise alleles is a technically 
      demanding challenge. Methods currently in use for HLA typing include 
      microcytotoxity assay, sequence-specific oligonucleotide (SSO) probe, 
      sequence-specific primer (SSP) amplification, and sequence-based typing (SBT). 
      Ambiguous results may still arise, even with the best molecular typing methods 
      but this problem can be resolved by using a combination of methods. It is clear 
      that a HLA typing laboratory will have to provide allele level resolution of HLA 
      data using the most appropriate means available and this will have the greatest 
      impact in terms of offering the best matched donor for the benefit of patients.
FAU - Chee, Ren Ee
AU  - Chee RE
AD  - Department of Microbiology, Faculty of Medicine, National University of 
      Singapore, Singapore.
LA  - eng
PT  - Journal Article
PT  - Review
PL  - Thailand
TA  - Southeast Asian J Trop Med Public Health
JT  - The Southeast Asian journal of tropical medicine and public health
JID - 0266303
SB  - IM
MH  - Alleles
MH  - Histocompatibility Testing/*methods
MH  - Humans
MH  - Sequence Analysis, DNA
RF  - 3
EDAT- 2003/05/21 05:00
MHDA- 2003/09/06 05:00
CRDT- 2003/05/21 05:00
PHST- 2003/05/21 05:00 [pubmed]
PHST- 2003/09/06 05:00 [medline]
PHST- 2003/05/21 05:00 [entrez]
PST - ppublish
SO  - Southeast Asian J Trop Med Public Health. 2002;33 Suppl 2:112-4.