PMID- 12756593
OWN - NLM
STAT- MEDLINE
DCOM- 20040202
LR  - 20181130
IS  - 0179-0358 (Print)
IS  - 0179-0358 (Linking)
VI  - 19
IP  - 6
DP  - 2003 Aug
TI  - Alterations in cholinergic and neuropeptide innervation of urinary bladder 
      following partial bladder outlet obstruction.
PG  - 427-31
AB  - Posterior urethral valves (PUV) are the most common cause of bladder outlet 
      obstruction (BOO) in infancy. Bladder instability, poor compliance and myogenic 
      failure are responsible for the poor long-term prognosis in these patients. 
      Previous studies have reported abundance of sensory neuropeptides, e.g. substance 
      P (SP), calcitonin gene-related peptide (CGRP), vasoactive intestinal polypeptide 
      (VIP) and acetylcholinesterase (AchE) nerves in the urinary bladder. We 
      hypothesized that the functional changes in the bladder following BOO are due to 
      alteration in cholinergic and sensory neuropeptide innervation. We therefore 
      investigated cholinergic and sensory innervation of urinary bladder following 
      BOO. Fifteen immature male guinea pigs (Hartley strain) 3-4 weeks old and 
      weighing approximately 250 g. underwent placement of a silk ligature around the 
      bladder neck to induce BOO. Controls included 5 sham-operated animals. The 
      animals were killed 1, 2 and 4 weeks following obstruction, respectively. 
      Whole-mount preparation and conventional sections of bladder wall were performed. 
      AchE histochemistry, and single-label immunofluorescence histochemistry for SP, 
      CGRP and VIP were utilized. Light microscopy and laser scanning confocal 
      microscopy were used to assess the results. AchE staining showed marked increase 
      in cholinergic innervation density within the suburothelial region following BOO. 
      The staining for SP, CGRP and VIP demonstrated marked reduction in sensory nerve 
      density within the suburothelial region 1 week following BOO and the lack of 
      sensory innervation 4 weeks after BOO. The marked reduction in sensory 
      innervation of the bladder and simultaneous increase in cholinergic innervation 
      following BOO may lead to bladder instability and decrease in bladder compliance.
FAU - Chertin, B
AU  - Chertin B
AD  - Children's Research Centre, Our Lady's Hospital for Sick Children, University 
      College Dublin, Dublin, Ireland.
FAU - Rolle, U
AU  - Rolle U
FAU - Cascio, S
AU  - Cascio S
FAU - Puri, P
AU  - Puri P
LA  - eng
PT  - Journal Article
DEP - 20030517
PL  - Germany
TA  - Pediatr Surg Int
JT  - Pediatric surgery international
JID - 8609169
RN  - 33507-63-0 (Substance P)
RN  - 37221-79-7 (Vasoactive Intestinal Peptide)
RN  - JHB2QIZ69Z (Calcitonin Gene-Related Peptide)
SB  - IM
MH  - Animals
MH  - Calcitonin Gene-Related Peptide/metabolism
MH  - Cholinergic Fibers/*metabolism
MH  - Fluorescent Antibody Technique
MH  - Guinea Pigs
MH  - Male
MH  - Microscopy, Confocal
MH  - Substance P/metabolism
MH  - Urinary Bladder/*innervation
MH  - Urinary Bladder Neck Obstruction/*physiopathology
MH  - Vasoactive Intestinal Peptide/metabolism
EDAT- 2003/05/21 05:00
MHDA- 2004/02/03 05:00
CRDT- 2003/05/21 05:00
PHST- 2002/07/10 00:00 [accepted]
PHST- 2003/05/21 05:00 [pubmed]
PHST- 2004/02/03 05:00 [medline]
PHST- 2003/05/21 05:00 [entrez]
AID - 10.1007/s00383-002-0937-6 [doi]
PST - ppublish
SO  - Pediatr Surg Int. 2003 Aug;19(6):427-31. doi: 10.1007/s00383-002-0937-6. Epub 
      2003 May 17.