PMID- 12757453
OWN - NLM
STAT- MEDLINE
DCOM- 20031009
LR  - 20240109
IS  - 0105-4538 (Print)
IS  - 0105-4538 (Linking)
VI  - 58
IP  - 6
DP  - 2003 Jun
TI  - Lettuce anaphylaxis: identification of a lipid transfer protein as the major 
      allergen.
PG  - 511-7
AB  - BACKGROUND: Allergy to plant-derived foods is associated with birch pollinosis in 
      central and northern Europe. Symptoms elicited are usually limited to the 
      oropharyngeal system. By contrast, in the Mediterranean area, allergy to the same 
      foods manifests more frequently with systemic reactions caused by nonspecific 
      lipid transfer proteins (nsLTP), independently of an associated pollinosis. 
      OBJECTIVE: We sought to investigate the pattern of immunoglobulin E (IgE) binding 
      protein bands implicated in lettuce allergy, in particular the presence of an 
      nsLTP. METHODS: Consecutive lettuce allergic patients were selected. 
      Determination of serum-specific IgE, immunoblot, and inhibition experiments were 
      performed in order to study the pattern of IgE binding proteins and the potential 
      cross-reactivity to pollens. Inhibition studies with recombinant allergens were 
      conducted to identify the lettuce allergens. The major allergen was subjected to 
      N-terminal amino acid sequencing. RESULTS: Fourteen patients were diagnosed as 
      being allergic to lettuce. All were sensitized to Platanus pollen. Ten of them 
      showed specific IgE to a lettuce protein of 9-kDa. The IgE binding to this 
      protein was completely inhibited by the cherry-LTP and peach extract. The 
      N-terminal sequence of the 9-kDa protein showed a high degree of amino acid 
      sequence identity to other nsLTPs. A clear partial cross-reactivity was observed 
      between lettuce-LTP and Platanus-pollen extract. CONCLUSIONS: An LTP has been 
      demonstrated to be a major allergen in patients suffering from lettuce allergy.
FAU - San Miguel-Moncin, M
AU  - San Miguel-Moncin M
AD  - Allergy Department, Institut Universitari Dexeus, Barcelona, Spain.
FAU - Krail, M
AU  - Krail M
FAU - Scheurer, S
AU  - Scheurer S
FAU - Enrique, E
AU  - Enrique E
FAU - Alonso, R
AU  - Alonso R
FAU - Conti, A
AU  - Conti A
FAU - Cistero-Bahima, A
AU  - Cistero-Bahima A
FAU - Vieths, S
AU  - Vieths S
LA  - eng
PT  - Journal Article
PL  - Denmark
TA  - Allergy
JT  - Allergy
JID - 7804028
RN  - 0 (Allergens)
RN  - 0 (Antigens, Plant)
RN  - 0 (Carrier Proteins)
RN  - 0 (Plant Proteins)
RN  - 0 (lipid transfer proteins, plant)
SB  - IM
MH  - Adult
MH  - Allergens/immunology/metabolism
MH  - Amino Acid Sequence/genetics
MH  - Anaphylaxis/*etiology
MH  - Antigens, Plant
MH  - Carrier Proteins/genetics/immunology/metabolism
MH  - Cross Reactions
MH  - Female
MH  - Food Hypersensitivity/*complications
MH  - Humans
MH  - Lactuca/*adverse effects/metabolism
MH  - Male
MH  - Plant Proteins
MH  - Pollen/immunology
MH  - Sequence Homology, Amino Acid
MH  - Trees/immunology
EDAT- 2003/05/22 05:00
MHDA- 2003/10/10 05:00
CRDT- 2003/05/22 05:00
PHST- 2003/05/22 05:00 [pubmed]
PHST- 2003/10/10 05:00 [medline]
PHST- 2003/05/22 05:00 [entrez]
AID - 097 [pii]
AID - 10.1034/j.1398-9995.2003.00097.x [doi]
PST - ppublish
SO  - Allergy. 2003 Jun;58(6):511-7. doi: 10.1034/j.1398-9995.2003.00097.x.