PMID- 12759103
OWN - NLM
STAT- MEDLINE
DCOM- 20040206
LR  - 20191025
IS  - 0890-6238 (Print)
IS  - 0890-6238 (Linking)
VI  - 17
IP  - 3
DP  - 2003 May-Jun
TI  - Evaluation of an in utero through lactational exposure protocol for detection of 
      estrogenic effects of ethinyl estradiol on the offspring of rats: preliminary 
      trial.
PG  - 335-43
AB  - As a preliminary trial of an in utero through lactational exposure protocol, 
      ethinyl estradiol, 0, 0.5, 5, or 50 micro g/kg/day, was administered by gavage to 
      pregnant Crj: CD (SD) IGS BR rats from gestational day (GD) 7 to day 18 after 
      delivery to evaluate the efficacy of this protocol and to estimate optimal 
      endpoints. The dams showed no abnormalities. Cleft phallus was observed in female 
      offspring at 50 micro g/kg. Other than a retardation of body weight gain in both 
      sexes at 50 micro g/kg/day, no other abnormal findings were detected. The fact 
      that cleft phallus was the only change induced suggests that the protocol is 
      applicable to the detection of effects of estrogenic chemicals given to pregnant 
      rats on offspring development and that the morphology of the female external 
      genitalia may be a useful endpoint.
FAU - Sawaki, Masakuni
AU  - Sawaki M
AD  - Chemicals Evaluation and Research Institute, 3-822, Ishii-machi, Hita, Oita 
      877-0061, Japan. sawaki-masakuni@cerij.jp
FAU - Noda, Shuji
AU  - Noda S
FAU - Muroi, Takako
AU  - Muroi T
FAU - Mitoma, Hideo
AU  - Mitoma H
FAU - Takakura, Saori
AU  - Takakura S
FAU - Sakamoto, Satoko
AU  - Sakamoto S
FAU - Yamasaki, Kanji
AU  - Yamasaki K
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Reprod Toxicol
JT  - Reproductive toxicology (Elmsford, N.Y.)
JID - 8803591
RN  - 0 (Estrogens)
RN  - 423D2T571U (Ethinyl Estradiol)
SB  - IM
MH  - *Abnormalities, Drug-Induced
MH  - Administration, Oral
MH  - Animals
MH  - Animals, Newborn
MH  - Cleft Palate/chemically induced
MH  - Dose-Response Relationship, Drug
MH  - Endpoint Determination/methods
MH  - Estrogens/*toxicity
MH  - Ethinyl Estradiol/*toxicity
MH  - Female
MH  - Genitalia, Female/abnormalities
MH  - *Lactation
MH  - Male
MH  - Pregnancy
MH  - *Prenatal Exposure Delayed Effects
MH  - Rats
MH  - Rats, Inbred Strains
EDAT- 2003/05/22 05:00
MHDA- 2004/02/10 05:00
CRDT- 2003/05/22 05:00
PHST- 2003/05/22 05:00 [pubmed]
PHST- 2004/02/10 05:00 [medline]
PHST- 2003/05/22 05:00 [entrez]
AID - S0890623803000054 [pii]
AID - 10.1016/s0890-6238(03)00005-4 [doi]
PST - ppublish
SO  - Reprod Toxicol. 2003 May-Jun;17(3):335-43. doi: 10.1016/s0890-6238(03)00005-4.