PMID- 12759924
OWN - NLM
STAT- MEDLINE
DCOM- 20030728
LR  - 20061115
IS  - 1045-2257 (Print)
IS  - 1045-2257 (Linking)
VI  - 37
IP  - 3
DP  - 2003 Jul
TI  - Heterogeneous pattern of chromosomal breakpoints involving the MYC locus in 
      multiple myeloma.
PG  - 261-9
AB  - Chromosomal rearrangements of the MYC locus, which often involve the IG loci, are 
      recurrent events in multiple myeloma (MM) and plasma cell leukemia (PCL). We used 
      dual-color fluorescence in situ hybridization (FISH) to characterize the 
      breakpoint locations of chromosomal translocations/rearrangements involving the 
      MYC locus at 8q24 found in a panel of 14 MM cell lines and 70 primary tumors (66 
      MM and 4 PCL). MYC locus alterations were observed in 21 cases: MYC/IG (mainly 
      IGH@) fusions in 11 cell lines and three patients (2 MM and 1 PCL), and extra 
      signals and/or abnormal MYC localizations in seven patients (5 MM and 2 PCL). 
      Fourteen of these cases were investigated by FISH analyses by use of a panel of 
      BAC clones covering about 6 Mb encompassing the MYC locus. The breakpoints were 
      localized in a region 100-250 kb centromeric to MYC in four cases, a region 
      500-800 kb telomeric to the gene in four cases, and regions > or = 2 Mb 
      centromeric or telomeric to MYC in five cases. Two different breakpoints were 
      detected in the KMS-18 cell line, whereas the insertion of a MYC allele was found 
      in a complex t(16;22) chromosomal translocation in the RPMI8226 cell line. Our 
      data document a relatively high dispersion of 8q24 breakpoints in MM.
CI  - Copyright 2003 Wiley-Liss, Inc.
FAU - Fabris, Sonia
AU  - Fabris S
AD  - Laboratorio di Ematologia Sperimentale e Genetica Molecolare, U.O. Ematologia 1, 
      Dipartimento di Scienze Mediche, Universita degli Studi di Milano, Ospedale 
      Maggiore IRCCS, Italy.
FAU - Storlazzi, Clelia Tiziana
AU  - Storlazzi CT
FAU - Baldini, Luca
AU  - Baldini L
FAU - Nobili, Lucia
AU  - Nobili L
FAU - Lombardi, Luigia
AU  - Lombardi L
FAU - Maiolo, Anna Teresa
AU  - Maiolo AT
FAU - Rocchi, Mariano
AU  - Rocchi M
FAU - Neri, Antonino
AU  - Neri A
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Genes Chromosomes Cancer
JT  - Genes, chromosomes & cancer
JID - 9007329
RN  - 0 (Genetic Markers)
RN  - 0 (Oncogene Proteins, Fusion)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Cell Line, Transformed
MH  - Chromosome Breakage/*genetics
MH  - Chromosomes, Human, Pair 8/genetics
MH  - Female
MH  - Gene Rearrangement/genetics
MH  - Genes, myc/*genetics
MH  - *Genetic Heterogeneity
MH  - Genetic Markers/*genetics
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Male
MH  - Middle Aged
MH  - Multiple Myeloma/*genetics/pathology
MH  - Oncogene Proteins, Fusion/genetics
MH  - Translocation, Genetic/genetics
MH  - Tumor Cells, Cultured
EDAT- 2003/05/22 05:00
MHDA- 2003/07/29 05:00
CRDT- 2003/05/22 05:00
PHST- 2003/05/22 05:00 [pubmed]
PHST- 2003/07/29 05:00 [medline]
PHST- 2003/05/22 05:00 [entrez]
AID - 10.1002/gcc.10211 [doi]
PST - ppublish
SO  - Genes Chromosomes Cancer. 2003 Jul;37(3):261-9. doi: 10.1002/gcc.10211.