PMID- 12759925
OWN - NLM
STAT- MEDLINE
DCOM- 20030728
LR  - 20061115
IS  - 1045-2257 (Print)
IS  - 1045-2257 (Linking)
VI  - 37
IP  - 3
DP  - 2003 Jul
TI  - Cytogenetic, spectral karyotyping, fluorescence in situ hybridization, and 
      comparative genomic hybridization characterization of two new secondary leukemia 
      cell lines with 5q deletions, and MYC and MLL amplification.
PG  - 270-81
AB  - Cytogenetic studies of patients with therapy-induced acute myeloid leukemia 
      (t-AML) have demonstrated whole chromosome loss or q-arm deletion of chromosomes 
      5 and/or 7 in a majority of cases. We have established two cell lines, SAML-1 and 
      SAML-2, from two patients who developed t-AML after radiation and chemotherapy 
      for Hodgkin disease. In both cases, the leukemia cells contained 5q deletions. 
      SAML-1 has 58 chromosomes and numerous abnormalities, including 
      der(1)(1qter-->1p22::5q31-->5qter), der(5)(5pter-->5q22::1p22-->1pter), +8, 
      der(13)i(13)(q10)del(13)(q11q14.1), and t(10;11). Fluorescence in situ 
      hybridization (FISH) with unique sequence probes for the 5q31 region showed loss 
      of IL4, IL5, IRF1, and IL3, and translocation of IL9, DS5S89, EGR1, and CSFIR to 
      1p. SAML-2 has 45 chromosomes, del(5)(q11.2q31) with a t(12;13)ins(12;5), leading 
      to the proximity of IRF1 and RB1, and complex translocations of chromosomes 8 and 
      11, resulting in amplification of MYC and MLL. Comparative genomic hybridization 
      and spectral karyotyping were consistent with the G-banding karyotype and FISH 
      analyses. Because a potential tumor suppressor(s) in the 5q31 region has yet to 
      be identified, these cell lines should prove useful in the study of the 
      mechanisms leading to the development of t-AML.
CI  - Copyright 2003 Wiley-Liss, Inc.
FAU - Knutsen, Turid
AU  - Knutsen T
AD  - Genetics Branch, National Cancer Institute, National Institutes of Health, 
      Bethesda, Maryland 20892, USA. knutsent@mail.nih.gov
FAU - Pack, Svetlana
AU  - Pack S
FAU - Petropavlovskaja, Maria
AU  - Petropavlovskaja M
FAU - Padilla-Nash, Hesed
AU  - Padilla-Nash H
FAU - Knight, Clement
AU  - Knight C
FAU - Mickley, Lyn A
AU  - Mickley LA
FAU - Ried, Thomas
AU  - Ried T
FAU - Elwood, Patrick C
AU  - Elwood PC
FAU - Roberts, Susan J
AU  - Roberts SJ
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PL  - United States
TA  - Genes Chromosomes Cancer
JT  - Genes, chromosomes & cancer
JID - 9007329
SB  - IM
EIN - Genes Chromosomes Cancer. 2003 Jul;37(3):332
MH  - Acute Disease
MH  - Adult
MH  - Chromosome Banding
MH  - Chromosome Painting
MH  - *Cytogenetic Analysis
MH  - Hodgkin Disease/*complications/drug therapy/radiotherapy
MH  - Humans
MH  - *In Situ Hybridization, Fluorescence
MH  - Karyotyping/methods
MH  - Leukemia, Myeloid/complications/*genetics
MH  - Leukemia, Radiation-Induced/*genetics
MH  - Male
MH  - Middle Aged
MH  - Nucleic Acid Hybridization/methods
MH  - Phenotype
MH  - Tumor Cells, Cultured
EDAT- 2003/05/22 05:00
MHDA- 2003/07/29 05:00
CRDT- 2003/05/22 05:00
PHST- 2003/05/22 05:00 [pubmed]
PHST- 2003/07/29 05:00 [medline]
PHST- 2003/05/22 05:00 [entrez]
AID - 10.1002/gcc.10200 [doi]
PST - ppublish
SO  - Genes Chromosomes Cancer. 2003 Jul;37(3):270-81. doi: 10.1002/gcc.10200.