PMID- 12760262
OWN - NLM
STAT- MEDLINE
DCOM- 20030612
LR  - 20190607
IS  - 1226-3303 (Print)
IS  - 2005-6648 (Electronic)
IS  - 1226-3303 (Linking)
VI  - 18
IP  - 1
DP  - 2003 Mar
TI  - Changes in serum cytokine concentration: a morphological study of liver cirrhosis 
      induced by common bile duct ligation in rats.
PG  - 6-12
AB  - BACKGROUND: Liver cirrhosis is a diffuse hepatic fibrosis, and nodule formation. 
      The transforming growth factor-beta 1 (TGF-beta 1) and interleukin-10 (IL-10) are 
      very important cytokines in hepatic fibrogenesis. The aim of this study was to 
      examine the relationship between the changes of the serum cytokines and 
      morphological changes following common bile duct ligation in experimental rats. 
      METHODS: Common bile ducts of fifty male Sprague-Dawley rats were ligated and 
      seven male rats were set aside as controls. Five rats each were sacrificed in 1, 
      2, 4, 6, 8, and 10 experimental weeks. Light microscopic studies and liver 
      function tests were performed during the above experimental weeks. The levels of 
      serum TGF-beta 1 and IL-10 were analyzed by ELISA. Also, alpha smooth muscle 
      actin (alpha-SMA) immunohistochemical stains were performed. RESULTS: On the 
      eighth week after common bile duct ligation, most hepatic lobular areas had been 
      replaced by proliferated bile ducts and fibrous tissue (typical biliary 
      cirrhosis). Serum TGF-beta 1 levels between the control group and the common bile 
      duct ligation group showed statistically significant changes. The alpha-SMA was 
      stained at proliferated bile ducts. These findings were correlated with each 
      other. CONCLUSION: Thus, this experiment may clarify our understanding of the 
      mechanism in liver fibrogenesis. Also, indicated is a need to explore the 
      therapeutic potential of these cytokines as anti-fibrotic agents.
FAU - Lee, Byung Seok
AU  - Lee BS
AD  - Department of Internal Medicine, Pathology, Chung Nam National University 
      Hospital, Daesa-dong, Jung-gu, Daejeon 301-721, Korea. gie001@cnuh.co.kr
FAU - Kim, Nam Jae
AU  - Kim NJ
FAU - Jeong, Hyun Yong
AU  - Jeong HY
FAU - Lee, Heon Young
AU  - Lee HY
FAU - Kang, Dae Young
AU  - Kang DY
FAU - Noh, Seung Moo
AU  - Noh SM
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PL  - Korea (South)
TA  - Korean J Intern Med
JT  - The Korean journal of internal medicine
JID - 8712418
RN  - 0 (Actins)
RN  - 0 (Cytokines)
RN  - 0 (Tgfb1 protein, rat)
RN  - 0 (Transforming Growth Factor beta)
RN  - 0 (Transforming Growth Factor beta1)
RN  - 130068-27-8 (Interleukin-10)
SB  - IM
MH  - Actins/analysis
MH  - Animals
MH  - Biopsy, Needle
MH  - Common Bile Duct/surgery
MH  - Cytokines/*blood
MH  - Disease Models, Animal
MH  - Immunohistochemistry
MH  - Interleukin-10/analysis
MH  - Ligation/methods
MH  - Liver Cirrhosis, Experimental/*pathology
MH  - Liver Function Tests
MH  - Liver Regeneration/physiology
MH  - Male
MH  - Random Allocation
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Sensitivity and Specificity
MH  - Transforming Growth Factor beta/analysis
MH  - Transforming Growth Factor beta1
PMC - PMC4531599
EDAT- 2003/05/23 05:00
MHDA- 2003/06/13 05:00
PMCR- 2003/03/01
CRDT- 2003/05/23 05:00
PHST- 2003/05/23 05:00 [pubmed]
PHST- 2003/06/13 05:00 [medline]
PHST- 2003/05/23 05:00 [entrez]
PHST- 2003/03/01 00:00 [pmc-release]
AID - kjim-18-1-6-2 [pii]
AID - 10.3904/kjim.2003.18.1.6 [doi]
PST - ppublish
SO  - Korean J Intern Med. 2003 Mar;18(1):6-12. doi: 10.3904/kjim.2003.18.1.6.