PMID- 12760476 OWN - NLM STAT- MEDLINE DCOM- 20030812 LR - 20181113 IS - 0830-9000 (Print) IS - 0830-9000 (Linking) VI - 67 IP - 2 DP - 2003 May TI - Comparative serologic and virologic study of commercial swine herds with and without postweaning multisystemic wasting syndrome. PG - 114-20 AB - A comparative serologic and virologic study was performed in pigs from 5 herds with postweaning multisystemic wasting syndrome (PMWS) and 2 herds without PMWS in Quebec. In each herd, 60 blood samples were collected at 4-wk intervals from pigs from 3 to 23 wk of age. The serum was evaluated for the presence of antibodies to porcine circovirus type 2 (PCV2) and porcine reproductive and respiratory syndrome virus (PRRSV), as well as for the presence of nucleic acid of PCV2, PRRSV, an porcine parvovirus (PPV), by means of the polymerase chain reaction (PCR). Serologic profiles for PCV2 were very similar in 6 of the 7 herds, including the 2 without PMWS, and were characterized by a gradual decrease in antibody titres from 3 until 11 wk of age, followed by seroconversion at 15 wk, and high PCV2 antibody titres thereafter in all pigs. Only starting at 11 to 15 wk of age could PCV2 viremia be detected, except in 1 herd, in which clinical signs were observed at 6 to 7 wk of age. A PCV2 viremia could be detected within the same pigs for a minimum of 8 wk, and the virus could still be detected in 41% of the serum samples obtained at 23 wk of age. The antibody level did not appear to influence the occurrence of disease, since titres were similar in pigs in the herds with or without PMWS. Infection with PRRSV, as demonstrated by PCR and seroconversion, preceded that of PCV2 by at least 1 mo in both types of herd. Both PRRSV and PCV2 were detected in some pigs in 5 of the 7 herds, including 1 herd without PMWS. Porcine parvovirus could be detected in serum by PCR in 2 herds with PMWS after the onset of clinical signs and also in 1 herd without PMWS. Genomic analysis of PCV2 strains identified in the herds without PMWS indicated complete or very high homology (99.4% to 100%) with the PCV2 strains identified in 4 herds with PMWS. In our field study, the triggering of PMWS in the herds could not be linked to coinfection with either PRRSV or PPV or to the use of a specific immunostimulant, such as vaccines, or to particular genomic differences between the PCV2 strains identified. FAU - Larochelle, Renee AU - Larochelle R AD - Laboratoire d'hygiene veterinaire et alimentaire, Agence canadienne d'inspection des aliments, 3400 Casavant ouest, St-Hyacinthe, Quebec J2S 8E3. larocheller@inspection.gc.ca FAU - Magar, Ronald AU - Magar R FAU - D'Allaire, Sylvie AU - D'Allaire S LA - eng PT - Comparative Study PT - Journal Article PT - Research Support, Non-U.S. Gov't PL - Canada TA - Can J Vet Res JT - Canadian journal of veterinary research = Revue canadienne de recherche veterinaire JID - 8607793 RN - 0 (Antibodies, Viral) RN - 0 (DNA, Viral) SB - IM MH - Amino Acid Sequence MH - Animals MH - Antibodies, Viral/blood MH - Base Sequence MH - Case-Control Studies MH - Circoviridae Infections/blood/*veterinary/virology MH - Circovirus/immunology/*isolation & purification MH - DNA, Viral/chemistry/genetics MH - Parvoviridae Infections/blood/*veterinary/virology MH - Parvovirus, Porcine/immunology/*isolation & purification MH - Polymerase Chain Reaction/veterinary MH - Quebec MH - Sequence Homology, Amino Acid MH - Sequence Homology, Nucleic Acid MH - Swine MH - Swine Diseases/blood/immunology/*virology MH - Wasting Syndrome/blood/*veterinary/virology MH - Weaning PMC - PMC227038 EDAT- 2003/05/23 05:00 MHDA- 2003/08/13 05:00 PMCR- 2003/08/01 CRDT- 2003/05/23 05:00 PHST- 2003/05/23 05:00 [pubmed] PHST- 2003/08/13 05:00 [medline] PHST- 2003/05/23 05:00 [entrez] PHST- 2003/08/01 00:00 [pmc-release] AID - 0000000-200305000-00006 [pii] PST - ppublish SO - Can J Vet Res. 2003 May;67(2):114-20.