PMID- 12760793
OWN - NLM
STAT- MEDLINE
DCOM- 20030908
LR  - 20121115
IS  - 0253-9624 (Print)
IS  - 0253-9624 (Linking)
VI  - 37
IP  - 1
DP  - 2003 Jan
TI  - [Brain-derived neurotrophic factor mRNA expressions in rat brain induced by 
      short-term in vivo exposure to deltamethrin].
PG  - 33-6
AB  - OBJECTIVE: To elucidate the mechanism of damage on central nervous system (CNS) 
      caused by deltamethrin (DM). METHODS: The mRNA and protein expressions of 
      brain-derived neurotrophic factor (BDNF) in the cerebral cortex and hippocampus 
      of the rats exposed to DM were measured by retro-transcription-polymerase chain 
      reaction (RT-PCR), dot blot, flow cytometry analysis and immunohistochemistry. 
      RESULTS: After exposure to DM at high-dose (DM1, 25.0 mg x kg(-1) x d(-1), i.p.) 
      once and low-dose (DM2, 12.5 mg x kg(-1) x d(-1), i.p.) for 5 days, the level of 
      BDNF mRNA and protein expression in the cerebral cortex and hippocampus of the 
      rats increased significantly. The levels of BDNF mRNA and protein expression in 
      the cerebral cortex and hippocampus measured by of RT-PCR in the rats with DM1 
      and DM2 were higher than those in the controls by 48% and 56%, and 59% and 54%, 
      respectively. And, those measured by dot blot in the rats with MD1 and MD2 were 
      186% and 161%, and 148% and 158% of those in the controls, respectively, 
      basically similar to those measured by RT-PCR. Flow cytometric analysis showed 
      that the levels of BDNF mRNA and protein expression in the cerebral cortex and 
      hippocampus in the rats with DM1 and DM2 were higher than those in the controls 
      by 53% and 89%, and 45% and 46%, respectively. Immunohistochemical analysis 
      showed that protein expression in the cerebral cortex of the rats with DM1 and 
      DM2 were 129% and 147% of those in the controls, same as the flow cytometric 
      analysis, but those were significantly higher in the hippocampus mainly in the 
      CA1 and DG areas of the rats with MD1 and the CA3 and DG areas of the rats with 
      DM2. CONCLUSIONS: DM could induce BDNF mRNA and protein expression in the 
      cerebral cortex and hippocampus of the rats, which could play an important role 
      in repairing of nerve damage.
FAU - Wang, Su-qing
AU  - Wang SQ
AD  - Department of Hygienic Toxicology, Tongji Medical College, Huazhong University of 
      Science and Technology, Wuhan 430030, China.
FAU - Shi, Nian
AU  - Shi N
FAU - Zhou, Li
AU  - Zhou L
FAU - Wu, You-tong
AU  - Wu YT
FAU - Li, Tao
AU  - Li T
FAU - Dong, Jie
AU  - Dong J
FAU - Chen, Liang
AU  - Chen L
LA  - chi
PT  - English Abstract
PT  - Journal Article
PL  - China
TA  - Zhonghua Yu Fang Yi Xue Za Zhi
JT  - Zhonghua yu fang yi xue za zhi [Chinese journal of preventive medicine]
JID - 7904962
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Insecticides)
RN  - 0 (Nitriles)
RN  - 0 (Pyrethrins)
RN  - 0 (RNA, Messenger)
RN  - 2JTS8R821G (decamethrin)
SB  - IM
MH  - Animals
MH  - Brain-Derived Neurotrophic Factor/*biosynthesis/genetics
MH  - Cerebral Cortex/*drug effects/metabolism
MH  - Hippocampus/*drug effects/metabolism
MH  - Insecticides/*toxicity
MH  - Male
MH  - Nitriles
MH  - Pyrethrins/*toxicity
MH  - RNA, Messenger/biosynthesis/drug effects
MH  - Rats
MH  - Rats, Wistar
MH  - Reverse Transcriptase Polymerase Chain Reaction
EDAT- 2003/05/23 05:00
MHDA- 2003/09/10 05:00
CRDT- 2003/05/23 05:00
PHST- 2003/05/23 05:00 [pubmed]
PHST- 2003/09/10 05:00 [medline]
PHST- 2003/05/23 05:00 [entrez]
PST - ppublish
SO  - Zhonghua Yu Fang Yi Xue Za Zhi. 2003 Jan;37(1):33-6.