PMID- 12761116
OWN - NLM
STAT- MEDLINE
DCOM- 20030619
LR  - 20210526
IS  - 0019-9567 (Print)
IS  - 1098-5522 (Electronic)
IS  - 0019-9567 (Linking)
VI  - 71
IP  - 6
DP  - 2003 Jun
TI  - Differential induction of interleukin-10 and interleukin-12 in dendritic cells by 
      microbial toll-like receptor activators and skewing of T-cell cytokine profiles.
PG  - 3337-42
AB  - Dendritic cells (DCs) discriminate different microbial pathogens and induce 
      T-cell responses of appropriate effector phenotypes accordingly. Microbial 
      recognition and differentiation are mediated in part by pattern recognition 
      receptors such as Toll-like receptors (TLRs), whereas the development of T-cell 
      effector functions is critically dependent on DC-derived cytokines such as 
      interleukin-12 (IL-12) and IL-10. However, it is not entirely clear to what 
      extent various microbial TLR activators could induce different functional states 
      of DCs that favor different T-cell effector phenotypes. Toward a better 
      understanding of this issue, we examined IL-10 and IL-12 production and 
      T-cell-polarizing potentials of murine bone marrow-derived DCs after stimulation 
      by three microbial TLR activators, namely, lipopolysaccharide (LPS), 
      peptidoglycan (PGN), and zymosan. We found that the three stimuli induced 
      drastically different profiles of IL-10 and IL-12 production in DCs. Further, 
      these stimuli differentially conditioned CD40-dependent IL-10 and IL-12 
      production by DCs. Finally, LPS-, PGN-, and zymosan-stimulated DCs primed 
      distinct T-cell cytokine profiles. Our results support the notion that 
      microbe-specific information sensed through different TLRs by DCs is linked to 
      differential Th priming through DC-derived cytokines.
FAU - Qi, Hai
AU  - Qi H
AD  - Department of Pathology, University of Texas Medical Branch, Galveston 
      77555-1070, USA.
FAU - Denning, Timothy L
AU  - Denning TL
FAU - Soong, Lynn
AU  - Soong L
LA  - eng
GR  - AI07626/AI/NIAID NIH HHS/United States
GR  - AI43003/AI/NIAID NIH HHS/United States
GR  - T32 AI007626/AI/NIAID NIH HHS/United States
GR  - R01 AI043003/AI/NIAID NIH HHS/United States
GR  - R56 AI043003/AI/NIAID NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Infect Immun
JT  - Infection and immunity
JID - 0246127
RN  - 0 (CD40 Antigens)
RN  - 0 (Cytokines)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (Membrane Glycoproteins)
RN  - 0 (Peptidoglycan)
RN  - 0 (Receptors, Cell Surface)
RN  - 0 (Toll-Like Receptors)
RN  - 130068-27-8 (Interleukin-10)
RN  - 187348-17-0 (Interleukin-12)
RN  - 9010-72-4 (Zymosan)
SB  - IM
MH  - Animals
MH  - CD40 Antigens/physiology
MH  - Cytokines/*biosynthesis
MH  - Dendritic Cells/drug effects/*immunology
MH  - Female
MH  - Interleukin-10/*biosynthesis
MH  - Interleukin-12/biosynthesis
MH  - Lipopolysaccharides/*pharmacology
MH  - Membrane Glycoproteins/*physiology
MH  - Mice
MH  - Peptidoglycan/*pharmacology
MH  - Receptors, Cell Surface/*physiology
MH  - T-Lymphocytes/*immunology
MH  - Toll-Like Receptors
MH  - Zymosan/*pharmacology
PMC - PMC155753
EDAT- 2003/05/23 05:00
MHDA- 2003/06/20 05:00
PMCR- 2003/06/01
CRDT- 2003/05/23 05:00
PHST- 2003/05/23 05:00 [pubmed]
PHST- 2003/06/20 05:00 [medline]
PHST- 2003/05/23 05:00 [entrez]
PHST- 2003/06/01 00:00 [pmc-release]
AID - 1634 [pii]
AID - 10.1128/IAI.71.6.3337-3342.2003 [doi]
PST - ppublish
SO  - Infect Immun. 2003 Jun;71(6):3337-42. doi: 10.1128/IAI.71.6.3337-3342.2003.