PMID- 12761444
OWN - NLM
STAT- MEDLINE
DCOM- 20040223
LR  - 20071115
IS  - 1234-1010 (Print)
IS  - 1234-1010 (Linking)
VI  - 9
IP  - 5
DP  - 2003 May
TI  - Protein kinase A expression and its possible roles in regulating tooth eruption 
      genes in the dental follicle.
PG  - BR160-7
AB  - BACKGROUND: Tooth eruption requires the chronological expression of a series of 
      genes in the dental follicle (DF). Protein kinase A (PKA) is a major 
      phosphorylation pathway in the cells, and may regulate the expression of tooth 
      eruption genes. MATERIAL/METHODS: In vivo, we studied the expression of the 
      regulatory (R) and catalytic (C) subunits of PKA in the DF of newborn rats. In 
      vitro, dental follicle cells (DFC) were treated with a specific PKA inhibitor, 
      and then the gene expression of monocyte chemotactic protein-1 (MCP-1), 
      colony-stimulating factor-1 (CSF-1) and parathyroid hormone-related protein 
      receptor (PTHrP-R) was determined. Cells also were treated with either 
      phorbol-12-myristate-13-acetate or dibutyryl-cAMP, and the gene expression for RI 
      alpha, RI beta, RII alpha and RII beta of PKA was examined. RESULTS: The results 
      indicate that RI alpha of PKA is the predominant subunit in the DF with steady 
      expression from days 1 to 11 postnatally. In contrast, expression of the RI beta, 
      RII alpha, RII beta subunits are progressively reduced over this time period. 
      However, there is a sharp decline of RI beta expression at postnatal day 3. The 
      expression of the C subunits slightly decreases at days 3 and 5 with a greater 
      decrease at day 7 postnatally. The specific PKA inhibitor reduces MCP-1 gene 
      expression and translation, as well as moderately reducing CSF-1 and PTHrP-R 
      expression. CONCLUSIONS: The reduction of the RI beta subunit in the rat DF at 
      day 3 may result in an elevated PKA activity to trigger the maximal burst of gene 
      expression of MCP-1 and CSF-1 seen at this time.
FAU - Yao, Shaomian
AU  - Yao S
AD  - Department of Comparative Biomedical Sciences, School of Veterinary Medicine, 
      Louisiana State University, Baton Rouge, Louisiana 70803, USA.
FAU - Wise, Gary E
AU  - Wise GE
LA  - eng
GR  - DE08911/DE/NIDCR NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Med Sci Monit
JT  - Medical science monitor : international medical journal of experimental and 
      clinical research
JID - 9609063
RN  - 0 (Chemokine CCL2)
RN  - 0 (Protein Subunits)
RN  - 0 (Receptor, Parathyroid Hormone, Type 1)
RN  - 81627-83-0 (Macrophage Colony-Stimulating Factor)
RN  - EC 2.7.11.11 (Cyclic AMP-Dependent Protein Kinases)
SB  - IM
MH  - Animals
MH  - Animals, Newborn
MH  - Cells, Cultured
MH  - Chemokine CCL2/genetics/metabolism
MH  - Cyclic AMP-Dependent Protein Kinases/chemistry/genetics/*metabolism
MH  - Dental Sac/growth & development/metabolism
MH  - Gene Expression Regulation, Developmental
MH  - Macrophage Colony-Stimulating Factor/genetics/metabolism
MH  - Protein Subunits
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Receptor, Parathyroid Hormone, Type 1/genetics/metabolism
MH  - Tooth Eruption/*genetics
EDAT- 2003/05/23 05:00
MHDA- 2004/02/24 05:00
CRDT- 2003/05/23 05:00
PHST- 2003/05/23 05:00 [pubmed]
PHST- 2004/02/24 05:00 [medline]
PHST- 2003/05/23 05:00 [entrez]
AID - 3068 [pii]
PST - ppublish
SO  - Med Sci Monit. 2003 May;9(5):BR160-7.