PMID- 12761877
OWN - NLM
STAT- MEDLINE
DCOM- 20031112
LR  - 20031114
IS  - 0730-2312 (Print)
IS  - 0730-2312 (Linking)
VI  - 89
IP  - 3
DP  - 2003 Jun 1
TI  - Identification, characterization, and expression of a novel alpha-tropomyosin 
      isoform in cardiac tissues in developing chicken.
PG  - 427-39
AB  - Tropomyosins are present in various muscle (skeletal, cardiac, and smooth) and 
      non-muscle cells with different isoforms characteristic of specific cell types. 
      We describe here a novel smooth/striated chimeric isoform that was expressed in 
      developing chick heart in addition to the classically described TM-4 type. This 
      novel alpha-Tm tropomyosin isoform, designated as alpha-Tm-2, contains exon 2a 
      (in place of exon 2b). The known striated muscle isoform (alpha-Tm-1) was also 
      expressed in embryonic hearts along with the striated muscle isoform of TM-4. In 
      adult heart, TM-4 was expressed, however, expression of both alpha-Tm-1 and 
      alpha-Tm-2 isoforms was drastically reduced or downregulated. Interestingly, we 
      were unable to detect the expression of alpha-Tm-2 in embryonic and adult 
      skeletal muscle, however, the alpha-Tm-1 isoform is expressed in embryonic and 
      adult skeletal muscle. Examination of other possible isoforms of the alpha-TM 
      gene, i.e., alpha-smooth muscle tropomyosin (alpha-Sm), alpha-Fibroblast-1 
      (alpha-F1), and alpha-Fibroblast-2 (alpha-F2) revealed expression in embryonic 
      hearts and a significant reduction of each of these isoforms in adult heart. In 
      order to elucidate the role of the newly discovered tropomyosin isoform in 
      chicken, we ectopically expressed the GFP fusion protein of alpha-Tm-1 and 
      alpha-Tm-2 separately into cardiomyocytes isolated from neonatal rats. Each 
      isoform was incorporated into organized myofibrils. Our results suggest that the 
      alpha-TM gene may undergo both positive and negative transcriptional control in 
      chicken hearts during development.
CI  - Copyright 2003 Wiley-Liss, Inc.
FAU - Zajdel, Robert W
AU  - Zajdel RW
AD  - Department of Cell and Developmental Biology, Syracuse, New York, USA.
FAU - Denz, Christopher R
AU  - Denz CR
FAU - Lee, Sung
AU  - Lee S
FAU - Dube, Syamalima
AU  - Dube S
FAU - Ehler, Elisabeth
AU  - Ehler E
FAU - Perriard, Evylene
AU  - Perriard E
FAU - Perriard, Jean-Claude
AU  - Perriard JC
FAU - Dube, Dipak K
AU  - Dube DK
LA  - eng
PT  - Journal Article
PL  - United States
TA  - J Cell Biochem
JT  - Journal of cellular biochemistry
JID - 8205768
RN  - 0 (Recombinant Fusion Proteins)
RN  - 0 (Tropomyosin)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - Chick Embryo
MH  - Chickens
MH  - Microscopy, Confocal
MH  - Molecular Sequence Data
MH  - Myocardium/*metabolism
MH  - Rats
MH  - Recombinant Fusion Proteins/metabolism
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Sequence Homology, Amino Acid
MH  - Transfection
MH  - Tropomyosin/chemistry/genetics/*metabolism
EDAT- 2003/05/23 05:00
MHDA- 2003/11/13 05:00
CRDT- 2003/05/23 05:00
PHST- 2003/05/23 05:00 [pubmed]
PHST- 2003/11/13 05:00 [medline]
PHST- 2003/05/23 05:00 [entrez]
AID - 10.1002/jcb.10504 [doi]
PST - ppublish
SO  - J Cell Biochem. 2003 Jun 1;89(3):427-39. doi: 10.1002/jcb.10504.