PMID- 12761976
OWN - NLM
STAT- MEDLINE
DCOM- 20040304
LR  - 20061115
IS  - 0412-3948 (Print)
IS  - 0412-3948 (Linking)
VI  - 36
IP  - 2
DP  - 2001 Apr
TI  - [Expression of BDNF and FGF-2 following axotomy in rat facial motoneurons].
PG  - 112-5
AB  - OBJECTIVE: To study the expression and distribution of brain derived neurotrophic 
      factor (BDNF) and fibroblast growth factor-2 (FGF-2) in normal facial motoneurons 
      (FMNs) and in FMNs following axotomy. METHODS: The right facial nerves were 
      transected 6 mm distal to the stylomastoid foraman in adult Wistar rats except 
      the normal group. Serial 20 microns cryosections were cut through the whole 
      brainstems. Expressions of BDNF and FGF-2 mRNA as well as BDNF and FGF-2 protein 
      were studied by in situ hybridization, immunohistochemistry and image analysis. 
      RESULTS: BDNF mRNA and its protein were observed in widespread areas of normal 
      rat facial nucleus, and those increased 1 day after axotomy. In addition to 
      neurons, glial cells were also stained. FGF-2 mRNA and its protein were mainly 
      localized in normal FMNs of ventral facial nucleus. After axotomy, expression of 
      FGF-2 mRNA started to up-regulate in FMNs at 7 days, however FGF-2 protein 
      drastically reduced at 3 and 7 days. CONCLUSION: In addition to 
      target-supporting, there may be BDNF autocrine and paracrine mechanisms as well 
      as FGF-2 autocrine mechanisms in normal rat FMN. When target-derived BDNF is 
      deprived, alternative sources of BDNF support may substitute immediately after 
      axotomy. However, deprivating of target--derived FGF-2 may result in 
      down-regulation of FGF-2 protein in adult rat FMNs at early stage after axotomy. 
      These suggest that the exogenous FGF-2 might provide a supportive environment for 
      the recovery of metabolism and function of FMNs at early stage following axotomy.
FAU - Qin, Z
AU  - Qin Z
AD  - Department of Otorhinolaryngology, First Affiliated Hospital of Henan Medical 
      University, Zhengzhou 450052, China. qinzhb@371.net
FAU - Dong, M
AU  - Dong M
LA  - chi
PT  - English Abstract
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Zhonghua Er Bi Yan Hou Ke Za Zhi
JT  - Zhonghua er bi yan hou ke za zhi
JID - 16210350R
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (RNA, Messenger)
RN  - 103107-01-3 (Fibroblast Growth Factor 2)
SB  - IM
MH  - Animals
MH  - Axotomy
MH  - Brain-Derived Neurotrophic Factor/*biosynthesis/genetics
MH  - Facial Nerve/*physiology/ultrastructure
MH  - Fibroblast Growth Factor 2/*biosynthesis/genetics
MH  - Male
MH  - Motor Neurons/*metabolism/ultrastructure
MH  - Nerve Regeneration
MH  - RNA, Messenger/biosynthesis
MH  - Rats
MH  - Rats, Wistar
EDAT- 2003/05/24 05:00
MHDA- 2004/03/05 05:00
CRDT- 2003/05/24 05:00
PHST- 2003/05/24 05:00 [pubmed]
PHST- 2004/03/05 05:00 [medline]
PHST- 2003/05/24 05:00 [entrez]
PST - ppublish
SO  - Zhonghua Er Bi Yan Hou Ke Za Zhi. 2001 Apr;36(2):112-5.