PMID- 12761985
OWN - NLM
STAT- MEDLINE
DCOM- 20040304
LR  - 20061115
IS  - 0412-3948 (Print)
IS  - 0412-3948 (Linking)
VI  - 36
IP  - 2
DP  - 2001 Apr
TI  - [Detection of mutations in exon 7 of p53 from fresh tumor samples by 
      single-strand conformation polymorphism and direct DNA sequencing].
PG  - 142-5
AB  - OBJECTIVE: To detect mutations of exon 7 of p53 gene in laryngeal squamous cell 
      carcinoma (LSCC) and analyze the relationship between the mutation and LSCC. 
      METHODS: Fresh tumor samples from 60 LSCC patients were examined using polymerase 
      chain reaction-single-strand conformation polymorphism (PCR-SSCP) and direct DNA 
      sequencing. RESULTS: An extra band in SSCP 3 was shown in 25 out of 60 samples. 
      Exon 7 of p53 were detected by direct DNA sequencing in 8 samples taken at random 
      from 25 cases. In 8 cases, 1 base pair deletion CCC-->CC in 250 codon which may 
      result in frame shift mutation was shown. Simultaneously, in 3 cases, 
      transversion AGT-->GGG in 261 codon which may bring out missense mutation was 
      found. In 2 cases, 1 base pair deletion CGG-->GG in 248 codon was detected. In 
      another case, 1 base pair deletion AGG-->AG in 249 codon was detected. 
      Transversion ATC-->TTC in 254 codon was found in one of the cases. Transversion 
      GGT-->TCA in 262 codon was found in another case. CONCLUSION: The codon 248, 250, 
      261 may be "hot spots" in LSCC. Mutations and deletions in exon 7 of p53 gene 
      occurred frequently in LSCC.
FAU - Zhang, X
AU  - Zhang X
AD  - Department of Otorhinolaryngology, Fourth Affiliated Hospital, West China 
      University of Medical Sciences, Chengdu 610041, China. iooi@263.net
FAU - Fu, Y
AU  - Fu Y
FAU - Liu, S
AU  - Liu S
FAU - Liang, C
AU  - Liang C
LA  - chi
PT  - English Abstract
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Zhonghua Er Bi Yan Hou Ke Za Zhi
JT  - Zhonghua er bi yan hou ke za zhi
JID - 16210350R
SB  - IM
MH  - Adult
MH  - Aged
MH  - Carcinoma, Squamous Cell/*genetics
MH  - Female
MH  - Genes, p53/*genetics
MH  - Humans
MH  - Laryngeal Neoplasms/*genetics
MH  - Male
MH  - Middle Aged
MH  - *Point Mutation
MH  - Polymerase Chain Reaction
MH  - Polymorphism, Single-Stranded Conformational
MH  - Sequence Analysis, DNA
EDAT- 2003/05/24 05:00
MHDA- 2004/03/05 05:00
CRDT- 2003/05/24 05:00
PHST- 2003/05/24 05:00 [pubmed]
PHST- 2004/03/05 05:00 [medline]
PHST- 2003/05/24 05:00 [entrez]
PST - ppublish
SO  - Zhonghua Er Bi Yan Hou Ke Za Zhi. 2001 Apr;36(2):142-5.