PMID- 12763580
OWN - NLM
STAT- MEDLINE
DCOM- 20030718
LR  - 20231213
IS  - 0165-3806 (Print)
IS  - 0165-3806 (Linking)
VI  - 143
IP  - 1
DP  - 2003 Jun 12
TI  - Early fetal expression of GABA(B1) and GABA(B2) receptor mRNAs on the development 
      of the rat central nervous system.
PG  - 47-55
AB  - GABA(B) receptors are G-protein-coupled receptors that mediate slow onset and 
      prolonged effects of GABA in the central nervous system (CNS). While they appear 
      to influence developmental events, depending on where they are found at a 
      synapse, little, if anything, is known as to the expression of GABA(B1) and 
      GABA(B2) receptor mRNAs during the early developmental stages. We used in situ 
      hybridization and RNase protection assays (RPA) to investigate the early fetal 
      expression of GABA(B1) and GABA(B2) receptor mRNAs on the development of the rat 
      CNS. Our in situ studies defined a pattern of early and strong GABA(B1) receptor 
      mRNA expression in the spinal cord, medullar and cerebral cortex neuroepithelium 
      of discrete brain regions on gestational day (GD) 11.5. On GD 12.5, GABA(B1) 
      receptor mRNAs were found in the hippocampal formation, cerebral cortex, 
      intermediate and posterior neuroepithelium, and the pontine neuroepithelium of 
      whole brain. RPA results showed GABA(B1) receptor mRNA was intensely expressed on 
      GD 11.5 and GD 12.5, when it was first detected in the ganglia, thalamus, and 
      cerebellum. However, GABA(B2) receptor mRNA was not detected on GD 10.5, 11.5, or 
      12.5. We suggest that GABA(B1) receptor might have a role in the early fetal 
      brain and spinal cord during pre- and post-synaptogenesis, neuronal maturation, 
      proliferation, and migration, and may be more important than the GABA(B2) 
      receptor in the early development of the rat CNS.
FAU - Kim, Myeong Ok
AU  - Kim MO
AD  - Division of Life Science, College of Natural Sciences and Applied Life Science 
      (BK21), Gyeongsang National University, Gazwa-dong 900, Chinju, 660-701, South 
      Korea. mokim@nongae.gsnu.ac.kr
FAU - Li, Shupeng
AU  - Li S
FAU - Park, Moon Seok
AU  - Park MS
FAU - Hornung, Jean-Pierre
AU  - Hornung JP
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Brain Res Dev Brain Res
JT  - Brain research. Developmental brain research
JID - 8908639
RN  - 0 (Gabbr2 protein, rat)
RN  - 0 (RNA Probes)
RN  - 0 (RNA, Messenger)
RN  - 0 (Receptors, GABA)
RN  - 0 (Receptors, GABA-B)
SB  - IM
MH  - Animals
MH  - Animals, Newborn
MH  - Blotting, Northern/methods
MH  - Central Nervous System/embryology/*metabolism
MH  - Embryo, Mammalian
MH  - Female
MH  - *Gene Expression Regulation, Developmental
MH  - In Situ Hybridization/methods
MH  - Male
MH  - Pregnancy
MH  - RNA Probes/chemistry/metabolism
MH  - RNA, Messenger/biosynthesis
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Receptors, GABA/*biosynthesis/genetics
MH  - Receptors, GABA-B/*biosynthesis/genetics
MH  - Tissue Distribution
EDAT- 2003/05/24 05:00
MHDA- 2003/07/19 05:00
CRDT- 2003/05/24 05:00
PHST- 2003/05/24 05:00 [pubmed]
PHST- 2003/07/19 05:00 [medline]
PHST- 2003/05/24 05:00 [entrez]
AID - S0165380603000993 [pii]
AID - 10.1016/s0165-3806(03)00099-3 [doi]
PST - ppublish
SO  - Brain Res Dev Brain Res. 2003 Jun 12;143(1):47-55. doi: 
      10.1016/s0165-3806(03)00099-3.