PMID- 12764044 OWN - NLM STAT- MEDLINE DCOM- 20030701 LR - 20190513 IS - 1047-3211 (Print) IS - 1047-3211 (Linking) VI - 13 IP - 6 DP - 2003 Jun TI - Contact-associated neurite outgrowth and branching of immature cortical interneurons. PG - 677-83 AB - When juvenile interneurons arrive at the cortical environment following tangential migration, they are faced with the task of positioning themselves in cortical space in preparation for local circuit wiring. This includes integration into different cortical layers and cessation of migration at various positions to ensure adequate coverage. Little is known about the signals or mechanisms that initiate a conversion from the migratory phenotype to the arborization phenotype. This study looks at the immediate changes in interneuron morphology after culturing for 24 h in a three-dimensional collagen gel. Immature interneurons taken from different stages of corticogenesis showed increased neurite branching and outgrowth after interneuronal contacts were made. These responses were suppressed in the presence of Slit and brain-derived neurotrophic factor (BDNF) if the interneurons were sourced from early to mid-stages of corticogenesis. However, interneurons taken from the late period of corticogenesis responded to Slit and BDNF by increasing branching and neurite outgrowth. These results suggest an initial interneuronal cell contact as a stimulus for propagating neuronal arborization that may lead to the formation of inhibitory neuronal circuits. In addition, we have identified the late corticogenetic period when interneurons are most sensitive to the neurite promoting effects of Slit and BDNF. FAU - Sang, Qian AU - Sang Q AD - Brain Development Laboratory, The Howard Florey Institute, University of Melbourne, Parkville, 3010, Victoria, Australia. FAU - Tan, Seong-Seng AU - Tan SS LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't PL - United States TA - Cereb Cortex JT - Cerebral cortex (New York, N.Y. : 1991) JID - 9110718 RN - 0 (Brain-Derived Neurotrophic Factor) RN - 0 (Drosophila Proteins) RN - 0 (Nerve Tissue Proteins) RN - 0 (sli protein, Drosophila) SB - IM MH - Animals MH - Animals, Newborn MH - Brain-Derived Neurotrophic Factor/metabolism MH - Cell Adhesion/physiology MH - Cell Communication/physiology MH - Cell Differentiation/physiology MH - Cell Movement/physiology MH - Cells, Cultured MH - Cerebral Cortex/*cytology/*embryology/physiology MH - *Drosophila Proteins MH - Interneurons/*cytology/physiology MH - Mice MH - Mice, Inbred C57BL MH - Nerve Net/*cytology/physiology MH - Nerve Tissue Proteins/metabolism MH - Neural Pathways/cytology/physiology MH - Neurites/*physiology/ultrastructure EDAT- 2003/05/24 05:00 MHDA- 2003/07/02 05:00 CRDT- 2003/05/24 05:00 PHST- 2003/05/24 05:00 [pubmed] PHST- 2003/07/02 05:00 [medline] PHST- 2003/05/24 05:00 [entrez] AID - 10.1093/cercor/13.6.677 [doi] PST - ppublish SO - Cereb Cortex. 2003 Jun;13(6):677-83. doi: 10.1093/cercor/13.6.677.