PMID- 12764099
OWN - NLM
STAT- MEDLINE
DCOM- 20030625
LR  - 20220316
IS  - 1529-2401 (Electronic)
IS  - 0270-6474 (Print)
IS  - 0270-6474 (Linking)
VI  - 23
IP  - 10
DP  - 2003 May 15
TI  - Differential activation of extracellular signal-regulated protein kinase in 
      primary afferent neurons regulates brain-derived neurotrophic factor expression 
      after peripheral inflammation and nerve injury.
PG  - 4117-26
AB  - To investigate the intracellular signal transduction pathways involved in 
      regulating the gene expression of brain-derived neurotrophic factor (BDNF) in 
      primary afferent neurons, we examined the activation of extracellular 
      signal-regulated protein kinase (ERK) in dorsal root ganglion (DRG) neurons after 
      peripheral inflammation and sciatic nerve transection. Peripheral inflammation 
      induced an increase in the phosphorylation of ERK, mainly in tyrosine kinase 
      A-containing small-to-medium-diameter DRG neurons. The treatment of the 
      mitogen-activated protein kinase (MAPK) kinase 1/2 inhibitor U0126 reversed the 
      pain hypersensitivity and the increase in phosphorylated-ERK (p-ERK) and BDNF in 
      DRG neurons induced by complete Freund's adjuvant. On the other hand, axotomy 
      induced the activation of ERK mainly in medium-and large-sized DRG neurons and in 
      satellite glial cells. U0126 suppressed the axotomy-induced autotomy behavior and 
      reversed the increase in p-ERK and BDNF. The intrathecal application of nerve 
      growth factor (NGF) induced an increase in the number of p-ERK-and BDNF-labeled 
      cells, mainly small neurons, and the application of anti-NGF induced an increase 
      in p-ERK and BDNF in some medium-to-large-diameter DRG neurons. The activation of 
      MAPK in the primary afferents may occur in different populations of DRG neurons 
      after peripheral inflammation and axotomy, respectively, through alterations in 
      the target-derived NGF. These changes, including the changes in BDNF expression, 
      might be involved in the pathophysiological changes in primary afferent neurons.
FAU - Obata, Koichi
AU  - Obata K
AD  - Department of Anatomy and Neuroscience, Hyogo College of Medicine, Nishinomiya, 
      Hyogo 663-8501, Japan.
FAU - Yamanaka, Hiroki
AU  - Yamanaka H
FAU - Dai, Yi
AU  - Dai Y
FAU - Tachibana, Toshiya
AU  - Tachibana T
FAU - Fukuoka, Tetsuo
AU  - Fukuoka T
FAU - Tokunaga, Atsushi
AU  - Tokunaga A
FAU - Yoshikawa, Hideki
AU  - Yoshikawa H
FAU - Noguchi, Koichi
AU  - Noguchi K
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Neurosci
JT  - The Journal of neuroscience : the official journal of the Society for 
      Neuroscience
JID - 8102140
RN  - 0 (Activating Transcription Factor 3)
RN  - 0 (Atf3 protein, rat)
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Butadienes)
RN  - 0 (Enzyme Inhibitors)
RN  - 0 (Glial Fibrillary Acidic Protein)
RN  - 0 (Neurofilament Proteins)
RN  - 0 (Nitriles)
RN  - 0 (Transcription Factors)
RN  - 0 (U 0126)
RN  - 108688-71-7 (neurofilament protein H)
RN  - EC 2.7.11.24 (Mitogen-Activated Protein Kinases)
SB  - IM
MH  - Activating Transcription Factor 3
MH  - Animals
MH  - Axotomy
MH  - Behavior, Animal/drug effects/physiology
MH  - Brain-Derived Neurotrophic Factor/*biosynthesis/genetics/immunology
MH  - Butadienes/pharmacology
MH  - Enzyme Activation/physiology
MH  - Enzyme Inhibitors/pharmacology
MH  - Ganglia, Spinal/*enzymology/injuries/metabolism
MH  - Gene Expression Regulation/drug effects/physiology
MH  - Glial Fibrillary Acidic Protein/metabolism
MH  - Immunohistochemistry
MH  - Inflammation/enzymology/physiopathology
MH  - Injections, Spinal
MH  - Male
MH  - Mitogen-Activated Protein Kinases/*metabolism/physiology
MH  - Neurofilament Proteins/metabolism
MH  - Neuroglia/drug effects/enzymology/metabolism/pathology
MH  - Neurons, Afferent/chemistry/*enzymology/metabolism/pathology
MH  - Nitriles/pharmacology
MH  - Pain Threshold/drug effects
MH  - Phosphorylation/drug effects
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Sciatic Nerve/enzymology/*injuries/*pathology
MH  - Transcription Factors/metabolism
PMC - PMC6741084
EDAT- 2003/05/24 05:00
MHDA- 2003/06/26 05:00
PMCR- 2003/11/15
CRDT- 2003/05/24 05:00
PHST- 2003/05/24 05:00 [pubmed]
PHST- 2003/06/26 05:00 [medline]
PHST- 2003/05/24 05:00 [entrez]
PHST- 2003/11/15 00:00 [pmc-release]
AID - 23/10/4117 [pii]
AID - 10.1523/JNEUROSCI.23-10-04117.2003 [doi]
PST - ppublish
SO  - J Neurosci. 2003 May 15;23(10):4117-26. doi: 10.1523/JNEUROSCI.23-10-04117.2003.