PMID- 12764114
OWN - NLM
STAT- MEDLINE
DCOM- 20030625
LR  - 20231013
IS  - 1529-2401 (Electronic)
IS  - 0270-6474 (Print)
IS  - 0270-6474 (Linking)
VI  - 23
IP  - 10
DP  - 2003 May 15
TI  - Osmotic regulation of estrogen receptor-beta in rat vasopressin and oxytocin 
      neurons.
PG  - 4261-9
AB  - The vasopressin (VP) magnocellular neurosecretory cells (MNCs) in the supraoptic 
      and paraventricular (PVN) nuclei are regulated by estrogen and exhibit robust 
      expression of estrogen receptor (ER)-beta. In contrast, only approximately 7.5% 
      of oxytocin (OT) MNCs express ER-beta. We examined the osmotic regulation of 
      ER-beta mRNA expression in MNCs using quantitative in situ hybridization 
      histochemistry. Hyper-osmolality induced via 2% hypertonic saline ingestion 
      significantly decreased, whereas sustained hypo-osmolality induced via 
      d-d-arginine VP and liquid diet increased ER-beta mRNA expression in MNCs (p < 
      0.05). Thus, the expression of ER-beta mRNA correlated inversely with changes in 
      plasma osmolality. Because hyper-osmolality is a potent stimulus for VP and OT 
      release, this suggests an inhibitory role for ER-beta in MNCs. 
      Immunocytochemistry demonstrated that the decrease in ER-beta mRNA was translated 
      into depletion of receptor protein content in hyper-osmotic animals. Numerous 
      MNCs were positive for ER-beta in control animals, but they were virtually devoid 
      of ER-beta-immunoreactivity (IR) in hyper-osmotic animals. The osmotically 
      induced decrease in ER-beta expression was selective for MNCs because ER-beta-IR 
      remained unaltered in PVN parvocellular neurons. Plasma estradiol and 
      testosterone were not correlated with ER-beta mRNA expression after osmotic 
      manipulation, suggesting that ER-beta expression was not driven by ligand 
      availability. Expression of FOS-IR in MNCs with attenuated ER-beta-IR, and the 
      absence of FOS-IR in parvocellular neurons that retain ER-beta-IR suggest a role 
      for neuronal activation in the regulation of ER-beta expression in MNCs. Thus, 
      osmotic modulation of ER-beta expression in MNCs may augment or attenuate an 
      inhibitory effect of gonadal steroids on VP release.
FAU - Somponpun, Suwit J
AU  - Somponpun SJ
AD  - Department of Physiology and Biophysics, University of Colorado Health Sciences 
      Center, Denver, Colorado 80262, USA.
FAU - Sladek, Celia D
AU  - Sladek CD
LA  - eng
GR  - R01 NS027975/NS/NINDS NIH HHS/United States
GR  - NS27975/NS/NINDS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Neurosci
JT  - The Journal of neuroscience : the official journal of the Society for 
      Neuroscience
JID - 8102140
RN  - 0 (Estrogen Receptor beta)
RN  - 0 (Hormones)
RN  - 0 (Receptors, Estrogen)
RN  - 11000-17-2 (Vasopressins)
RN  - 451W47IQ8X (Sodium Chloride)
RN  - 50-56-6 (Oxytocin)
SB  - IM
MH  - Animals
MH  - Blood Volume/physiology
MH  - Body Weight/physiology
MH  - Estrogen Receptor beta
MH  - Hematocrit
MH  - Hormones/blood/physiology
MH  - Hypernatremia/physiopathology
MH  - Hyponatremia/physiopathology
MH  - Male
MH  - Neurons/cytology/*metabolism
MH  - Osmolar Concentration
MH  - *Osmotic Pressure
MH  - Oxytocin/blood/*metabolism
MH  - Paraventricular Hypothalamic Nucleus/cytology/metabolism/physiology
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Receptors, Estrogen/biosynthesis/genetics/*metabolism
MH  - Sodium Chloride/metabolism
MH  - Supraoptic Nucleus/cytology/metabolism/physiology
MH  - Vasopressins/blood/*metabolism
MH  - Water-Electrolyte Balance/physiology
MH  - Water-Electrolyte Imbalance/physiopathology
PMC - PMC6741117
EDAT- 2003/05/24 05:00
MHDA- 2003/06/26 05:00
PMCR- 2003/11/15
CRDT- 2003/05/24 05:00
PHST- 2003/05/24 05:00 [pubmed]
PHST- 2003/06/26 05:00 [medline]
PHST- 2003/05/24 05:00 [entrez]
PHST- 2003/11/15 00:00 [pmc-release]
AID - 23/10/4261 [pii]
AID - 10.1523/JNEUROSCI.23-10-04261.2003 [doi]
PST - ppublish
SO  - J Neurosci. 2003 May 15;23(10):4261-9. doi: 10.1523/JNEUROSCI.23-10-04261.2003.