PMID- 12767009
OWN - NLM
STAT- MEDLINE
DCOM- 20030801
LR  - 20081121
IS  - 0146-6615 (Print)
IS  - 0146-6615 (Linking)
VI  - 70
IP  - 3
DP  - 2003 Jul
TI  - Interaction of human herpesvirus 6 with human CD34 positive cells.
PG  - 444-50
AB  - We reported previously that human herpesvirus 6 (HHV-6) suppresses hematopoietic 
      colony formation of erythroid (BFU-E), granulocyte/macrophage (CFU-GM), and 
      megakaryocyte (CFU-Meg) lineages in vitro. Here we describe the interaction 
      between HHV-6 and human CD34+ cells, which are a major source of hematopoietic 
      progenitor cells. CD34+ cells were immunomagnetically isolated from cord blood 
      mononuclear cells using anti-CD34+ antibodies coated onto either Dynabeads trade 
      mark or MACS beads. The CD34+ population selected with Dynabeads showed a broad 
      range of fluorescence. The population selected with MACS beads showed a narrow 
      range of fluorescence. After infection with HHV-6, two transcripts of the 
      immediate early genes were detected with both cell populations. HHV-6 suppressed 
      colony formation of BFU-E, CFU-GM, and CFU-Meg. HHV-6 suppressed cell growth 
      after 3 to 7 days culture in the presence of thrombopoietin (TPO). More 
      differentiated CD34+ cells were more susceptible to the effects of HHV-6. These 
      data indicate that the targets for hematopoietic suppression by HHV-6 are the 
      differentiated cells.
CI  - Copyright 2003 Wiley-Liss, Inc.
FAU - Isomura, Hiroki
AU  - Isomura H
AD  - Department of Virology, Okayama University Medical School, Okayama, Japan. 
      hiroki-isomura@uiowa.edu
FAU - Yoshida, Mariko
AU  - Yoshida M
FAU - Namba, Hikaru
AU  - Namba H
FAU - Yamada, Masao
AU  - Yamada M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Med Virol
JT  - Journal of medical virology
JID - 7705876
RN  - 0 (Antigens, CD34)
RN  - 0 (RNA, Messenger)
RN  - 0 (RNA, Viral)
SB  - IM
MH  - Antigens, CD34/analysis
MH  - Cells, Cultured
MH  - Colony-Forming Units Assay
MH  - Erythroid Precursor Cells/cytology
MH  - Fetal Blood/immunology
MH  - Granulocytes/cytology
MH  - Hematopoietic Stem Cells/cytology/immunology/*virology
MH  - Herpesvirus 6, Human/genetics/isolation & purification/*physiology
MH  - Humans
MH  - Macrophages/cytology
MH  - Megakaryocytes/cytology
MH  - RNA, Messenger/analysis
MH  - RNA, Viral/genetics
MH  - Reverse Transcriptase Polymerase Chain Reaction
EDAT- 2003/05/27 05:00
MHDA- 2003/08/02 05:00
CRDT- 2003/05/27 05:00
PHST- 2003/05/27 05:00 [pubmed]
PHST- 2003/08/02 05:00 [medline]
PHST- 2003/05/27 05:00 [entrez]
AID - 10.1002/jmv.10415 [doi]
PST - ppublish
SO  - J Med Virol. 2003 Jul;70(3):444-50. doi: 10.1002/jmv.10415.